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Method for promoting and controlling antibacterial effect of nano-silver

A nano-silver antibacterial and nano-silver technology is applied in botanical equipment and methods, chemicals for biological control, animal repellents, etc. It can solve the problems of no bactericidal effect and stimulate the growth of microorganisms, and achieve practicality. Powerful, simple operation, wide application effect

Inactive Publication Date: 2015-11-18
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, recent studies have shown that because low concentrations of nano-silver can activate cell repair, this repair effect overcompensates the toxicity of nano-silver, so that nano-silver not only does not have a bactericidal effect in the low concentration range, but will stimulate microorganisms. growth, which is a great challenge to the bactericidal application of nano-silver

Method used

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  • Method for promoting and controlling antibacterial effect of nano-silver
  • Method for promoting and controlling antibacterial effect of nano-silver
  • Method for promoting and controlling antibacterial effect of nano-silver

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Embodiment 1

[0025] A method for promoting and controlling nano-silver antibacterial effect of the present invention, comprising the following steps:

[0026] (1) Preparation of nano-silver solution:

[0027] Add 57.3mL ultrapure water into the beaker, and while stirring in an ice bath, add 1.50mL, 24mM sodium citrate solution and 1.0mL, 24mM sodium borohydride solution in turn, after stirring evenly, quickly add 0.55mL, 23.5 mM silver nitrate solution, the solution turns yellow, indicating that sodium citrate-nano-silver is generated, and sodium citrate is attached to the nano-silver. After stirring in an ice bath for 3 minutes, turn to normal temperature (25°C) and stir for 3 hours. After washing, centrifugation and purification, nano silver is obtained. Nano-silver was dissolved in ultrapure water to obtain a nano-silver solution with an initial concentration of 16.851mg / L.

[0028] (2) Preparation of nano-silver antibacterial agent: with reference to Table 1, the sodium hydrogen sulf...

Embodiment 2

[0035] A method for promoting and controlling nano-silver antibacterial effect of the present invention, comprising the following steps:

[0036] (1) Preparation of nano-silver solution: with embodiment 1.

[0037] (2) Preparation of nano-silver antibacterial agent: with reference to Table 1, the thioacetamide solution with an initial concentration of 10 mM was mixed evenly with the nano-silver solution prepared in step (1), and nano-silver antibacterial agents of different concentrations were prepared.

[0038] (3) Get the nano-silver antibacterial agent prepared in step (2) and spray it on the antibacterial area (containing the pathogenic bacteria area), make the pathogenic bacteria Escherichia coli soak in the nano-silver antibacterial agent for 12h, complete the antibacterial to pathogenic bacteria deal with.

[0039]Table 2 is the impact data of different concentrations of nano-silver antibacterial agents on the antibacterial effect. As can be seen from Table 2, the pre...

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Abstract

The invention discloses a method for promoting and controlling an antibacterial effect of nano-silver. The method comprises the steps that a sulfide solution and a nano-silver solution are evenly mixed to obtain a nano-silver antibacterial agent; the nano-silver antibacterial agent is sprayed to an area containing pathogenic bacteria, and treatment on the pathogenic bacteria is completed. According to the method for promoting and controlling the antibacterial effect of the nano-silver, due to the fact that a sulfide is added, the antibacterial effect of the nano-silver can be effectively promoted, and the growth stimulation effect produced in the sterilization process can be avoided; the antibacterial effect of the nano-silver can be effectively controlled by controlling the concentration of the sulfide; the method is simple, convenient and easy to carry out, the antibacterial effect of the nano-silver can be effectively promoted, and the occurrence of noneffective sterilization or even microbial growth promotion is avoided.

Description

technical field [0001] The invention belongs to the field of antibacterial application of nano silver, in particular to a method for promoting and controlling the antibacterial effect of nano silver by utilizing sulfide. Background technique [0002] NanoSilver (NanoSilver) is the metal silver whose particle size is nanoscale. The particle size of nano-silver is mostly around 25 nanometers, which has a strong inhibitory and killing effect on dozens of pathogenic microorganisms such as Escherichia coli, Neisseria gonorrhoeae, and Chlamydia trachomatis, and will not produce drug resistance. The research on nano-silver is currently mainly focused on the antibacterial properties of nano-silver. Its antibacterial characteristics include: broad-spectrum antibacterial, strong sterilization, strong permeability, repair and regeneration, long-lasting antibacterial, and no drug resistance. Due to these characteristics of nano-silver, It is widely used in modern medicine. [0003] Na...

Claims

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Application Information

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IPC IPC(8): A01N59/16A01P1/00
Inventor 郭志陈桂秋曾光明晏铭黄真真易峰何凯王佳佳
Owner HUNAN UNIV