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Chloroacetamide herbicide degrading strain, bacterium produced thereby and application thereof

A technology of chloroacetamide and herbicide, which is applied to the degrading strain of chloroacetamide herbicides and the bacterial agent produced by it and the application field, which can solve the problems of long time consumption, unfavorable application, and low degradation rate of acetochlor, etc. To achieve the effect of protecting the ecological environment, protecting the health, wide application potential and value

Active Publication Date: 2015-11-18
NANJING AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the degradation rate of acetochlor is low, it takes a long time, and the degradation experiment in soil has not been carried out, which is not conducive to the application in the field

Method used

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  • Chloroacetamide herbicide degrading strain, bacterium produced thereby and application thereof
  • Chloroacetamide herbicide degrading strain, bacterium produced thereby and application thereof
  • Chloroacetamide herbicide degrading strain, bacterium produced thereby and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Embodiment 1, isolation and identification of bacterial strain

[0031] Add 5 g of acetochlor-contaminated soil samples into 100 mL of liquid inorganic salt medium (hereinafter referred to as MM) containing 0.2 mM acetochlor, culture on a shaker at 30°C and 150 rpm for 7 days, and transfer to 5% inoculum size (v / v) In the same fresh medium, enrichment culture was carried out four times in succession. Dilute the fifth-generation enrichment solution on MM solid medium containing 0.2mM acetochlor, culture at 30°C for 5 days, pick a single colony on the plate, transfer it to a 3mL LB test tube for culture, and collect the bacteria by centrifugation. Transfer to 20mL MM medium containing 0.2mM acetochlor, culture at 30°C for 5 days, freeze-dry the culture solution and redissolve it in 5mL methanol, and use UV scanning and high performance liquid chromatography (HPLC) to detect the degradation effect of single colony , and finally isolated and screened an acetochlor-degradin...

Embodiment 2

[0033] Embodiment 2, laboratory degradation experiment

[0034] 2.1 Preparation of seed solution

[0035] Insert the strain AC‐1 into 100mL LB medium containing 0.2mM acetochlor, culture it on a shaker at 30°C and 150rpm, collect the bacteria by centrifugation at 6000rpm after 24h, wash the bacteria twice with MM, and finally resuspend them with 10mLMM, as The seed solution is ready for use.

[0036] 2.2 Degradation of chloroacetamide herbicides by strain AC‐1

[0037] Inoculate the strain AC-1 into 100mLMM containing 0.2mM alachlor, acetochlor, butachlor and metolachlor according to 5% inoculum amount, culture on a shaker at 30°C and 150rpm, every 8 hours Sampling 5mL, taken up to 48 hours. The residues of alachlor, acetochlor, butachlor and metolachlor were detected, the degradation rate was calculated, and the time-degradation curve of strain AC-1 to chloroacetamide herbicides was drawn. like figure 2 , the strain AC-1 can completely degrade 0.2mM acetochlor within 48h,...

Embodiment 3

[0047] Embodiment 3, soil degradation experiment

[0048] The vegetable garden soil was taken as the test soil sample. Pass the soil sample through a 2mm sieve, take a certain amount of alachlor, acetochlor and butachlor powder and dissolve them in 10mL of methanol, and then soak in diatomaceous earth to completely absorb the pesticide. The soaked diatomaceous earth is placed in a fume hood to dry, and it is mixed into the soil so that the concentration of the pesticide in the soil is about 1mg / kg. 500g of each soil sample was cultured in a constant temperature incubator at 30°C, and the inoculum was inserted into the seed solution at an inoculation amount of 10%, and the soil without inoculation was used as a control, and the water holding capacity of the soil was maintained at 60%. After culturing for 7 days, the residual amount was determined by HPLC. The measurement results are shown in Table 1.

[0049] It can be concluded from Table 1 that after 7 days of cultivation,...

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Abstract

The invention discloses a chloroacetamide herbicide degrading strain, a bacterium produced thereby and application thereof. The chloroacetamide herbicide degrading strain, rhodococcus sp. AC-1, is preserved in China General Microbiological Culture Collection Center under CGMCC No.10778 on 30th April, 2015. The chloroacetamide herbicide degrading strain AC-1 is suitable for use in degrading chloroacetamide herbicides, preferably in degrading chloroacetamide herbicides in soil; by directly applying a degrading strain product, residue of the chloroacetamide herbicides (specifically alachlor, acetochlor and butachlor) in the soil can be decreased by above 85%, the problem that the residue of the alachlor, acetochlor and butachlor in agricultural production is out of limits is solved, and chemical damage caused by herbicide residue to crops is prevented.

Description

technical field [0001] The invention belongs to the field of biological high technology, and relates to a chloroacetamide herbicide-degrading bacterial strain and the bacterial agent produced therefor and its application. technical background [0002] As an indispensable and important means of production in modern agriculture, chemical pesticides have played a vital role in protecting agricultural production and ensuring food security in my country and the world. However, due to the large number of people and the small land in our country, the multiple cropping index of farmland is high, and the use of chemical pesticides exceeds the standard, and due to the backward application technology, the utilization rate of pesticides is only about 20%-30%, and 70%-80% of pesticides enter the soil and water bodies, causing soil pollution. Serious pollution of pesticide residues in agricultural products and agricultural products endangers human health and ecological environment safety, ...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C12R1/01
Inventor 蒋建东洪青陈相艳陈蕾蕾刘孝永陈凯
Owner NANJING AGRICULTURAL UNIVERSITY
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