A kind of brevundimonas for heavy metal cadmium pollution control and its application
A technology of brevundimonas and heavy metals, which is applied in the field of environmental governance and microorganisms, can solve problems such as threats to the ecological environment, acute poisoning or death of humans, and achieve the effect of reducing biological toxicity
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Embodiment 1
[0014] Example 1 Isolation and identification of Brevundomonas H368
[0015] (1) Isolation of cadmium-resistant bacteria H368
[0016] A. Weigh 10g of cadmium-contaminated soil in 90mL of sterile physiological saline, shake it on a constant temperature shaker at 28°C, and after standing still, take the supernatant for culture;
[0017] B. Transfer the supernatant to sterile 0.5 times LB (containing 200 mg / L Cd 2+ ) In the culture medium, place it in a constant temperature shaker at 28°C for about 10 days, and then inoculate it to contain 300 mg / L Cd at 10% 2+ In 0.5 times of LB medium, cultured in a constant temperature shaker at 28°C for about 10 days, and then transferred to 400mg / L Cd in the same way. 2+ Cultured in 0.5 times LB medium for about 10 days. Then take 0.2ml of the culture and spread it onto the 200mg / L Cd 2+ In the 0.5 times LB solid plate, place it in a constant temperature incubator at 28°C until a single colony grows; pick a single colony to contain 200mg / L of Cd 2...
Embodiment 2
[0024] Example 2 Environmental adaptation characteristics of Brevundomonas H368
[0025] Brevundomonas H368's tolerance to multiple heavy metals and antibiotic tolerance test.
[0026] Inoculate H368 strain into fresh 0.5 times LB medium for activation overnight, then inoculate fresh 0.5 times LB medium with 1% (v / v) inoculum, and cultivate to OD in a constant temperature shaker at 28°C 600 =0.5; then inoculate the M9 medium containing different cadmium ion concentrations and 0.5 times the LB medium with 1% (v / v) inoculum, then put it in a constant temperature shaker at 28℃ for 3 days, then take Centrifuge 2ml of culture at 6000rpm for 5 minutes, add 2ml of sterile saline to suspend, measure OD 600 Absorbance value. At the same time, take 2ml of the culture from the culture, centrifuge at 6000rpm under aseptic conditions for 5 minutes, add 2ml of sterile normal saline to suspend, and then use sterile normal saline for gradient dilution, and then take 10 -3 ,10 -4 ,10 -5 Three dilut...
Embodiment 3
[0035] Example 3 Cadmium ion removal ability of Brevundomonas H368
[0036] Furthermore, the applicant tested different cadmium ions (Cd 2+ ) Growth status and cadmium ion adsorption capacity of Brevundomonas H368 under stress conditions.
[0037] Activate the H368 strain according to the method of Example 2, and then inoculate it with different Cd at a 1% (v / v) inoculation ratio 2+ Culture in LB medium of 0.5 times the concentration, 28°C, 180rpm shaker. Then take 2ml of culture at different times, centrifuge at 4℃, 5000rpm for 8min, discard the supernatant, then suspend it in 2ml of sterile saline, and then measure its OD 600 value. After culturing to 72 hours, take 10ml of the culture, centrifuge at 5000rpm for 8min at 4℃, take the supernatant out and send it to the analysis and testing center for cadmium ion concentration analysis; meanwhile, the precipitated part is still suspended in 10ml sterile saline, and then its OD is measured 600 value. Three parallel processes are set...
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