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Application method of applying vanillin to control root-knot nematode incognita under soil conditions

A technology of southern root knot nematode and an application method, which is applied in the field of plant protection using vanillin, can solve problems such as unclear effect of vanillin on nematodes, and achieve the effect of inhibiting infection and reproduction

Active Publication Date: 2018-07-17
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been preliminarily verified under indoor simulated conditions that vanillin has a strong effect of inhibiting nematode hatching and killing nematodes, but the effect of vanillin on controlling nematodes under complex soil conditions is not clear

Method used

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  • Application method of applying vanillin to control root-knot nematode incognita under soil conditions
  • Application method of applying vanillin to control root-knot nematode incognita under soil conditions
  • Application method of applying vanillin to control root-knot nematode incognita under soil conditions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1 is directly applied to the control effect of vanillin on root-knot nematode incognita

[0030] In the present embodiment, the cultivation and collection methods of root-knot nematode incognita are as follows:

[0031] Plant planting: Soak Jiafen No. 18 tomato seeds in 15% (V / V) hydrogen peroxide solution for 15 minutes to sterilize, wash with water, spread the seeds evenly in a petri dish covered with two layers of moist filter paper, and keep away from light The treatment was placed in a constant temperature culture room. After germination, it is sown on a seedling board containing vermiculite. After the seedlings grow to three leaves and one heart, they are transplanted into plastic pots filled with sterilized soil. Nematodes were inoculated 10 days after transplanting.

[0032] Nematode inoculation method: uniformly punch four 2-3cm deep holes at about 1cm at the base of the tomato stem, inject the suspension containing the second instar larvae of Mel...

Embodiment 2

[0037] Except that the vanillin concentration configured is 0.2mmol / kg·dry soil, other operations are the same as in Example 1.

Embodiment 3

[0039] Except that the vanillin concentration configured is 0.4mmol / kg·dry soil, other operations are the same as in Example 1.

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Abstract

The invention belongs to the field of agriculture and provides an application method for applying vanilline under soil conditions to prevent and treat meloidogyne incognita chitwood. The application method is used for applying the vanilline to soil. Particularly, at the seedling age of tomatoes with three leaves and one heart to tomatoes with five leaves and one heart, seedlings are transplanted, one seedling is planted in each pot, and the vanilline is applied to the soil after seedling transplantation, wherein an application mode is a direct application mode or a drop irrigation mode and by dry weight of the soil, the application amount of the vanilline is 0.2 to 8.0mmol / kg dry soil. According to the invention, the prevention and treatment effects of the vanilline on meloidogyne are observed and an experiment of applying the vanilline to the soil by adopting a direct application method and simulating a drop irrigation method so as to prevent and treat the meloidogyne incognita chitwood is performed. When the vanilline with concentration of 0.2 to 4.0mmol / kg dry soil is directly applied to the soil under the soil conditions, not only are normal growth and development of tomatoes ensured, but also infection and reproduction of the meloidogyne are inhibited, wherein the tomato root-knot number inhibition ratio reaches 26.5 percent to 57.2 percent and the egg mass inhibition ratio reaches 29.5 percent to 46.2 percent.

Description

technical field [0001] The invention belongs to the field of prevention and control of agricultural diseases and insect pests, and in particular relates to a plant protection method utilizing vanillin. Background technique [0002] Incognito root-knot nematode (Meloidogyne incognita) is one of the most important pathogenic organisms in the world, and it has brought serious economic losses to the world's agricultural production. Meloidogyne incognita has a wide host range, totaling more than 1700 plant species (Sasser et al., 1983. The international Meloidogyne project-its goals and accomplishments. Annual Review of Phytopathology 21, 271–288). Its life cycle length is mainly affected by temperature, humidity and host species. The soil temperature is 20-30°C, the relative humidity is 40%-70%, and the pH is 4-8, which is most conducive to the growth and reproduction of nematodes. Meloidogyne incognita only needs 30-60 days to complete a life cycle under normal temperature an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G13/00
CPCA01G13/00
Inventor 左元梅姚媛媛卢巧芳钟丽娜
Owner CHINA AGRI UNIV