Method for rapidly inducing rice blast for agar substrate seedling nursery

Abstract

The present invention discloses a method for rapidly inducing rice blasts for agar substrate seedling nursery. According to the method, rice is inoculated with pyricularia grisea at a rice sprout stage, and thus an inoculation morbidity result can be obtained within a short period. The method comprises a first step of preparing an assorted nutrient solution; a second step of preparing a seedling culturing agar substrate; a third step of seeding; a fourth step of culturing rice sprouts within a closed ecological niche; a fifth step of preparing an inoculant spore emulsion; a sixth step of inoculating the sprouts with spore emulsion; and a seventh step of cultivation management after the inoculation. The method is advantageous in that the whole method is short in time, only eight days being required from seeding to typical morbidity; important morbidity conditions such as temperature, humidity, water fertilizer and the ecological niche are highly consistent and are easy to control; a germfree seedling nursery environment is easy to create; and operation is easy to implement, the overall technology is simple, and standardization of the technical method is easy to realize.

Description

technical field [0001] The present invention relates to phytopathological techniques and microbiological techniques. Specifically, it is a method for rapidly inducing rice blast by growing seedlings on agar matrix. Background technique [0002] Rice blast is a major disease of rice production in my country. In a pandemic year, it caused a loss of 15 billion kilograms of rice grain to my country. Although the current understanding of rice blast is quite in-depth, a large amount of research investment is still needed to achieve sustainable, safe and efficient control of rice blast. However, many studies on rice blast are inseparable from the basic method of artificially induced disease. Even the research in the molecular field, such as the problem of genes related to pathogenic bacteria or genes related to host disease resistance, often requires the results of artificially induced diseases to answer. . So far, the technology of artificially induced disease related to rice b...

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Problems solved by technology

However, the currently widely used method of inducing disease at the seedling stage is to use the fully expanded seedling leaves to inoculate after the 3-5 leaf stage of the rice seedlings. It lasts more than 1 week, and the test cycle usually spans more than 3 weeks, which is suitable for some research work that requires short-term results, such as identification of pathogenic mutants in molecular plant disease research, expression of disease-resistant genes, and infection process It is still suspected that it takes too long to transmit information on the pathogen-host interaction; moreover, in order to obtain robust rice seedlings, sufficient sunlight and supplementary nutrients need to be provided, and the entire method system still needs to use a larger space such as a greenhouse ; The control of local conditions such as humidity, temperature, fertilizer and water during the test period also has great technical difficulties, or is quite limited by equipment conditions, etc.

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