A kind of ultrasonic extraction method of complete ladderane lipid in anammox bacteria
An anammox bacteria and extraction method technology are applied in the field of ultrasonic extraction of complete ladderane lipids in anammox bacteria, and can solve the problems of long cycle, high cost, complicated operation, etc., and achieve favorable release and stable biological activity of substances. , the effect of high-quality extraction
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[0042] Follow the steps below to extract complete ladderane lipids from anammox bacteria:
[0043] 1) Sampling: Take 15ml of sludge as a sample, the sludge is aqueous sludge containing anammox bacteria; remove the upper layer of water after centrifugation;
[0044] 2) Freeze-drying: Vacuum freeze-drying technology is used to remove interstitial moisture in the sample in step 1), that is, the sludge sample is processed with the freeze dryer of Beijing Boyikang Company. The parameters are set as follows: vacuum degree 0.08-0.09Mpa, temperature- 40°C; freeze-dry for 24 hours, and place the freeze-dried samples in a -20°C refrigerator for later use;
[0045] 3) Add the extract: put 8g of the dry sample obtained in step 2) into a 50ml glass centrifuge tube, and add 10mL of methanol, 5mL of dichloromethane and 4mL of phosphate buffer to form an extract, in which methanol, dichloromethane and The volume ratio of phosphate buffer is 2:1:0.8;
[0046] 4) Ultrasonic extraction: Set an...
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