Ultrasonic extraction method of complete ladderane lipid in anaerobic ammonium oxidation bacteria

Abstract

The invention discloses an ultrasonic extraction method of a complete ladderane lipid in anaerobic ammonium oxidation bacteria. The complete ladderane lipid is a special phosphatide existed on intracellular anaerobic ammonium oxidation body membranes of anaerobic ammonium oxidation bacteria. Research shows that complete ladderane lipid used as a research object can provide a new thinking and new support information for anaerobic ammonia oxidation research with important ecological and physiological research meaning. The operation flow of the method comprises: sampling-freeze drying-extract addition-ultrasonic extraction-extract component proportion adjustment-centrifugal separation-nitrogen sweeping condensation. After analysis of HPLC-MS, the method provided by the invention is proved to have good effect and high extraction purity.

Description

technical field [0001] The invention belongs to the technical field of organic matter extraction, and relates to an ultrasonic extraction method for complete ladderane lipids in anammox bacteria. Background technique [0002] Anaerobic ammonium oxidation bacteria can convert nitrite and ammonia nitrogen into nitrogen to shorten the process of ammonia nitrogen conversion, which has the advantages of high efficiency and low consumption. Therefore, the anaerobic ammonium oxidation process based on this type of microorganism has become a promising sewage denitrification process. . In order to better apply the anammox process to large-scale production, the biological research on anammox bacteria, especially the research based on intracellular chemical substances is very necessary. [0003] In the study, scholars discovered a special type of phospholipid structure on the membrane of the anammox body, the largest organelle in the anammox bacteria cell, whose function is to improve...

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Problems solved by technology

However, this type of method is complicated to operate and has a long period, and the purity of the extracted complete ladder is relatively low, which is not conducive to popularization and use.

[0006] At present, the complete ladder is regarded as a conventional phospholipid for extraction, and the extraction methods that can be used are mainly divided into two categories, one is physical extraction, such as using supercritical fluid technology, etc., but the cost of such methods is too high; the other is The first category is chemical extraction methods, such as organic solvent extraction, column chromatography, enzymatic hydrolysis and other methods. The extraction efficiency is different for different samples, so the stability of the extraction cannot be guaranteed.

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