Disodium pamidronate and glucose injection and preparation method thereof
A technology of disodium pamidronate and glucose injection, which is applied in the direction of medical formulas, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc. It can solve the problems of short curative effect maintenance time, curative effect decline, increased fever, etc. question
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Embodiment 1-4
[0017] Embodiment 1-4: Preparation of Pamidronate Disodium Glucose Injection
[0018] The prescription content of table 1 embodiment 1-4 pamidronate disodium glucose injection
[0019] Component content
[0020] Preparation process: add glucose and 2-aminoisobutyric acid to 80% water for injection, stir until dissolved, add 0.1% (W / V) activated carbon for needles, stir well, filter and decarbonize, and adjust the obtained filtrate to pH5 with phosphoric acid .5-6.5, then add Pamidil Disodium Phosphate, stir until Pamidil Disodium Phosphate is completely dissolved, add the remaining amount of water for injection to constant volume, sterilize and filter with a 0.22μm microporous membrane, fill, and sterilize with moist heat to obtain clarity solution.
Embodiment 5
[0021] Example 5: Experimental Study on the Effect of Drugs on Rat Breast Cancer Pain Model with Bone Metastasis
[0022] Take out the cryopreservation tube of breast cancer Walker256 cells, and put it into a 37°C water bath to quickly thaw. Wipe off the water stains on the cryopreservation tube, disinfect the nozzle with 75% alcohol by volume, unscrew the nozzle on the ultra-clean workbench, suck out the cell suspension with a straw, transfer it into a centrifuge tube, and then add an appropriate amount containing 10 RPMI-1640 medium with % fetal bovine serum, pipet to suspend the cells, centrifuge at 1000r / min for 5min, discard the supernatant, rinse with the medium repeatedly once, add an appropriate amount of medium, inoculate into a culture bottle, and place in an incubator After culturing, the medium was replaced once the next day to continue culturing, and the growth was observed. Cells in the logarithmic growth phase were selected and inoculated into the abdominal cavi...
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