Geotrichum candidum and application thereof in greenhouse soil remediation

A greenhouse and soil remediation technology, which is applied in the field of agricultural microbes to achieve the effects of improving and activating soil, fast growth rate, and strong growth and reproduction ability

Active Publication Date: 2015-12-16
新疆惠森生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are many reports on the study of the effects of organic materials on soil properties and crop yield in the field, the isolation and screening of strains that can improve greenhouse soil from greenhouse soil, and the use of self-selection to screen dome

Method used

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  • Geotrichum candidum and application thereof in greenhouse soil remediation
  • Geotrichum candidum and application thereof in greenhouse soil remediation
  • Geotrichum candidum and application thereof in greenhouse soil remediation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Isolation, screening and identification of Geotrichum Candidum XHS0030BCGMCCNO.9435

[0030] 1. Isolation and screening of strains

[0031] (1) Separation

[0032] The Geotrichum candidum used in the present invention is sampled and separated from typical greenhouse soils with serious salinization, sandy soil or protruding hardening in Korla, southern Xinjiang. A strain with a certain antagonistic ability to soil problems such as salinization and soil compaction. Use the traditional plate culture method to isolate the microorganisms in the soil layer, purify the strains by the plate streak method, and use different culture temperatures, pH values, and medium as enrichment conditions to screen out a batch of well-growing microbial strains, and select one of them The strain numbered XHS0030B.

[0033] Separation steps: according to the gradient dilution method, weigh 10g of soil samples in 90mL sterile saline, activate for 30min at 25°C and perform gradient ...

Embodiment 2

[0045] Example 2: Molecular level of Geotrichum Candidum XHS0030BCGMCCNO.9435

[0046] 1. PCR amplification of the ITSrDNA sequence of Geotrichum candidum and its sequencing

[0047] Pick a small amount of single colonies of the XHS0030B strain, put them into an EP tube filled with 25 μL of sterile water, boil at 100°C for 8-10 minutes, and then quickly put them into the ice-water mixture for 5 minutes. Centrifuge at 10000r / min for 5min, store at 4°C, and take the supernatant when used.

[0048] The construction of ITSrDNA gene sequence determination and phylogenetic tree thereof: extract the total DNA of bacterial strain according to routine method, carry out the PCR amplification of ITSrDNA section with deionized water with the dilution universal primer ITS1 and ITS4, primer design is as follows:

[0049] ITS1(F): 5'-TCCGTAGGTGAACCTGCGG-3'

[0050] ITS4(R): 5'-TCCTCCGCTTATTGATATGC-3'

[0051] The 50 μl reaction system contains: 5 μl of 10×PCR buffer, 20 pmol of each primer,...

Embodiment 3

[0054] Example 3: Preparation of greenhouse soil remediation agent

[0055] (1) Inoculation: prepare solid culture medium of four strains of Geotrichum candidum, Rhodopseudomonas palustris, Candida utilis and Mortierella alpina, perform strict aseptic operation after sterilization, and transfer from the inclined plane to the plate , cultured at 28°C for 3 days.

[0056] (2) Primary culture: Pick a single colony from the solid medium in step (1) and transfer it to a 50mL Erlenmeyer flask filled with liquid medium, perform aseptic operation, and culture at 25°C and 120r / min for 24h- 48h.

[0057] (3) Secondary fermentation: Inoculate the primary culture strains into a 500mL Erlenmeyer flask filled with liquid medium, perform aseptic operation, and culture at 25°C and 120r / min for 36h-72h.

[0058] (4) Compatibility of strain fermentation broth: 70-80 parts of Geotrichum candida secondary fermentation broth prepared in step (3), 30-50 parts of Rhodopseudomonas paluscans seconda...

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Abstract

The invention discloses geotrichum candidum and application thereof in greenhouse soil remediation. By carrying out strain isolating, screening, breeding and domesticating on a greenhouse soil sample as well as through determination of morphological characteristics and rDNA ITS sequence of the obtained strain and through phylogenetic analysis, the effect of the strain geotrichum candidum XHS0030B CGMCC NO.9435 in greenhouse soil remediation is determined; 70-80 parts of geotrichum candidum, 30-50 parts of rhodopseudomonas palustris, 20-40 parts of candida utilis and 10-30 parts of mortierella alpina are mixed, and a mixed strain liquid is fully adsorbed in a carrier and is mixed with accessories, wherein the carrier is selected from greenhouse soil and the accessories include urine and calcium superphosphate; a greenhouse soil remediation compound is prepared by virtue of a fermentation technology; and the soil remediation compound has an outstanding technical effect in greenhouse soil remediation.

Description

technical field [0001] The invention relates to the technical field of agricultural microorganism application, in particular, the invention relates to a self-selected Geotrichum candidum and the technical field of its application in greenhouse soil restoration. Background technique [0002] In intensive agricultural production, soil is a spatio-temporal continuum, and the spatial variability of soil properties must be considered in precise management. At present, due to the lack of necessary fertilization guidance for farmers, many farmers mostly manage fertilization based on their own experience, and believe that "high input, high output", which leads to a large number of blind excessive fertilization often occurs. However, although excessive fertilization can increase crop yields in the short term, in the long run, excessive fertilization will lead to a decrease in soil productivity, crop yield reduction, and the risk of groundwater pollution. [0003] Soil salinization i...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N1/20C12N1/16C09K17/00C12R1/645C12R1/72C12R1/01C09K101/00
Inventor 王心歌张松林韩友青
Owner 新疆惠森生物技术有限公司
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