Method for detecting melamine by adopting two-photon excited fluorescence

A two-photon excitation and melamine technology, which is applied in the field of chemical analysis, can solve the problems of difficulty in detecting melamine and achieve great application potential and a wide range of applications

Active Publication Date: 2015-12-16
YANCHENG INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0012] Purpose of the invention: The technical problem to be solved by the present invention is to provide a method for two-photon excited

Method used

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  • Method for detecting melamine by adopting two-photon excited fluorescence
  • Method for detecting melamine by adopting two-photon excited fluorescence
  • Method for detecting melamine by adopting two-photon excited fluorescence

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Step 1, preparation of gold nanoparticles

[0030] All glass instruments used were soaked in aqua regia, washed with distilled water, and dried for later use. 0.25mL concentration is 0.1mol / LHAuCl 4 The solution was added to 100 mL of deionized water and heated to boiling. Add 1.88mL of sodium citrate solution with a concentration of 0.1g / mL into the boiling liquid, the solution turns light blue after 5s and wine red after 1min. Continue to react for 15 min, stop stirring, and let stand to cool to room temperature. A gold nanoparticle solution with a concentration of 13nmol / L and a particle size of 15nm was obtained. TEM image of gold nanoparticles figure 1 shown.

[0031] Step 2, get the background signal

[0032] Take 0.2 mL of the gold nanoparticle solution prepared in the above steps and dilute it by 1 time with deionized water, add 0 mL of melamine, mix well, and let stand for 10 min. Then, take 0.1ml sample and dilute it with 0.38ml deionized water to obtai...

Embodiment 2

[0043] The standard curve was obtained according to the steps described in Example 1, except that the particle size of the gold nanoparticles was changed to 30 nm.

Embodiment 3

[0045] The standard curve was obtained according to the steps described in Example 1, except that the particle size of the gold nanoparticles was changed to 50 nm.

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Abstract

The invention discloses a method for detecting melamine by adopting two-photon excited fluorescence. According to the method disclosed by the invention, melamine is detected by using a two-photon excited fluorescence method, and two-photon excited fluorescence has deep penetrability, so that the method can be used for detecting melamine in a complex environment and is wide in application scope. The method has great application potential in detection of melamine in a complex environment, so that a new detection means can be provided for supervision on melamine.

Description

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Claims

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Application Information

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Owner YANCHENG INST OF TECH
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