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Preparation method of a label-free electrochemical immunosensor based on molybdenum disulfide/nickel-palladium alloy nanocomposite

A nanocomposite material and immunosensor technology, which is applied in scientific instruments, analytical materials, and material analysis through electromagnetic means, can solve the problems of cumbersome process and low yield, and achieve simple preparation, convenient operation, and improved electrochemical response The effect of signal speed

Active Publication Date: 2016-06-22
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, most of the synthesis methods are synthesized separately, and then compound the catalyst and the carrier, the process is cumbersome and the yield is not high

Method used

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  • Preparation method of a label-free electrochemical immunosensor based on molybdenum disulfide/nickel-palladium alloy nanocomposite

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 MoS 2 / NiPd preparation method

[0028] Take 35mL of CTAB solution with a concentration of 0.1mol / L, add 0.02g of NiCl 2 ·6H 2 O and 2 mL of H at a concentration of 0.03 mol / L 2 PdCl 4 solution, after stirring for 15 minutes, continue to stir and add 2 mL of ascorbic acid solution with a concentration of 0.3 mol / L, 0.5 mL of Na with a concentration of 0.1 mol / L 2 MoO 4 solution and 0.01 g of Na 2 S, after stirring for 15 minutes, put it into the reaction kettle, and react for 14 hours at 180°C; after cooling to room temperature, use deionized water to wash centrifugally, and vacuum dry at 40°C to obtain MoS 2 / NiPd, which was dissolved in deionized water to obtain MoS at a concentration of 1 mg / mL 2 / NiPd solution.

Embodiment 2

[0029] Example 2 MoS 2 / NiPd preparation method

[0030] Take 35mL of CTAB solution with a concentration of 0.1mol / L, add 0.04g of NiCl 2 ·6H 2 O and 4 mL of H at a concentration of 0.03 mol / L 2 PdCl 4 solution, after stirring for 15 minutes, continue to stir and add 4 mL of ascorbic acid solution with a concentration of 0.3 mol / L, 1.0 mL of Na with a concentration of 0.1 mol / L 2 MoO 4 solution and 0.02g Na 2 S, after stirring for 15 minutes, put it into the reaction kettle, and react for 16 hours at 150°C; after cooling to room temperature, use deionized water to wash centrifugally, and vacuum dry at 40°C to obtain MoS 2 / NiPd, which was dissolved in deionized water to obtain MoS at a concentration of 1 mg / mL 2 / NiPd solution.

Embodiment 3

[0031] Example 3 MoS 2 / NiPd preparation method

[0032] Take 35mL of CTAB solution with a concentration of 0.1mol / L, add 0.06g of NiCl 2 ·6H 2 O and 6 mL of H at a concentration of 0.03 mol / L 2 PdCl 4 solution, after stirring for 15 minutes, continue to stir and add 6 mL of ascorbic acid solution with a concentration of 0.3 mol / L, 1.5 mL of Na with a concentration of 0.1 mol / L 2 MoO 4 solution and 0.03g Na 2 S, after stirring for 15 minutes, put it into the reaction kettle, and react for 12 hours at 220°C; after cooling to room temperature, use deionized water to wash centrifugally, and vacuum dry at 40°C to obtain MoS 2 / NiPd, which was dissolved in deionized water to obtain MoS at a concentration of 1 mg / mL 2 / NiPd solution.

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Abstract

The invention discloses a preparation method of a label-free electrochemical immunosensor based on a molybdenum disulfide / nickel-palladium alloy nanocomposite material, and belongs to the field of new nanometer functional materials and biosensors. In the present invention, molybdenum disulfide / nickel-palladium alloy nano-composite material MoS2 / NiPd is firstly prepared by a one-pot method, and then a simple, fast and sensitive drug for the detection of gastric cancer tumor markers is prepared by utilizing its excellent adsorption and electrochemical catalytic properties. Label-free electrochemical immunosensor.

Description

technical field [0001] The invention relates to a preparation method of a label-free electrochemical immunosensor based on an inorganic nanocomposite material, and the prepared sensor can be used for detecting gastric cancer tumor markers. It belongs to the technical field of new nanometer functional materials and biosensors. Background technique [0002] One of the main methods for the diagnosis of gastric cancer patients is to detect gastric cancer tumor markers. Therefore, it is of great significance to develop sensitive and specific sensors for detecting gastric cancer tumor markers. [0003] Electrochemical immunosensors are widely used in the fields of clinical analysis and environmental detection due to their high sensitivity, good specificity, and easy operation. Among them, label-free electrochemical immunosensors have the advantages of relatively simple preparation and little environmental impact. Broader application prospects. To construct a label-free electroch...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327G01N33/574
Inventor 李娇娇张勇魏琴马洪敏吴丹
Owner UNIV OF JINAN