Method for preparing soybean-protein antibacterial agent

A technology of soybean protein and antibacterial agent, which is applied in the field of preparation of soybean protein antibacterial agent, can solve the problems of easy decomposition and poor heat resistance of organic antibacterial agent, and achieve the effect of improving antibacterial performance

Inactive Publication Date: 2015-12-23
高大元
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem mainly solved by the present invention: In the process of plastic processing and product preparation, when using the existing inorganic antibacterial agent, due to the high content of heavy metal ions, it is harmful to human health, while the organic antibacterial agent has poor heat resistance and is easy to decompose. The problem is to provide a method of alkaline hydrolysis of soybean protein, and at the same time, the alkaline hydrolysis product is enzymatically hydrolyzed into soybean polypeptide, which can effectively inhibit the growth and reproduction of bacteria after plastic production, and at the same time greatly improve the stability and action time of antibacterial agents.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0017] According to the ratio of defatted soybean powder and deionized water solid-to-liquid mass ratio of 1:10, at 20°C, stir at 800r / min for 25min to obtain soybean powder suspension, and adjust its pH to 4.0, continue stirring at 45°C for 10 minutes; after the stirring is completed, place it in a centrifuge at 3300r / min for 10 minutes, remove the lower layer of sediment, and then mix the sediment and deionized water at a solid-to-liquid mass ratio of 1:10 , and stir evenly, then centrifuge again at 3300-3500r / min for 10-15min and separate, repeat the above steps twice to obtain concentrated soybean protein, and then place it in an oven at 80°C for 10 hours to obtain concentrated soybean protein Powder; the concentrated soybean protein powder prepared above and 0.1mol / L sodium hydroxide solution, according to the solid-liquid mass ratio of 1:5 at 600r / min, stirred and mixed to disperse to form a soybean protein suspension, and then the soybean The protein suspension was heat...

example 2

[0019]According to the ratio of defatted soybean powder and deionized water solid-to-liquid mass ratio of 1:10, at 25°C, stir at 1000r / min for 27min to obtain soybean powder suspension, and adjust its pH to 4.2, continue stirring at 47°C for 12 minutes; after the stirring is completed, place it in a centrifuge at 3400r / min for 12 minutes, remove the lower layer of sediment, and then mix the sediment and deionized water at a solid-to-liquid mass ratio of 1:10 , and stir evenly, then centrifuge again at 3300-3500r / min for 10-15min and separate, repeat the above steps twice to obtain concentrated soybean protein, and then place it in an oven at 82°C for 11 hours to obtain concentrated soybean protein Powder; the concentrated soybean protein powder prepared above and 0.1mol / L sodium hydroxide solution, according to the solid-liquid mass ratio of 1:5 at 700r / min, stirred and mixed to disperse to form a soybean protein suspension, and then the soybean The protein suspension was heat...

example 3

[0021] According to the ratio of defatted soybean powder and deionized water solid-to-liquid mass ratio of 1:10, stir at 1200r / min for 30min at a temperature of 30°C to obtain a suspension of soybean powder, and adjust its pH to 4.5, continue stirring at 50°C for 15 minutes; after the stirring is completed, place it in a centrifuge at 3500r / min for 15 minutes, remove the lower layer of sediment, and then mix the sediment and deionized water at a solid-to-liquid mass ratio of 1:10 , and stir evenly, then centrifuge again at 3300-3500r / min for 10-15min and separate, repeat the above steps 3 times to obtain concentrated soybean protein, and then place it in an oven at 85°C for 12 hours to obtain concentrated soybean protein Powder; the concentrated soybean protein powder prepared above and 0.1mol / L sodium hydroxide solution, according to the solid-liquid mass ratio of 1:5 at 800r / min, stirred and mixed to disperse to form a soybean protein suspension, and then the soybean The pro...

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PUM

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Abstract

The invention relates to a method for preparing a soybean-protein antibacterial agent and belongs to the field of plastic additives. The method is used for solving the problems that the content of heavy metal ions in the existing inorganic antibacterial agents is relatively high, the product appearance is affected, and organic antibacterial agents are relatively poor in heat resistance and are prone to decomposition. The method comprises the steps of defatting soybeans so as to prepare concentrated soybean protein powder, carrying out alkali dipping on soybean protein so as to prepare a soybean suspension, carrying out enzymolysis on the soybean suspension through subtilisin so as to form a soybean protein polypeptide, preparing an antibacterial agent crude product, then, carrying out purifying by using sephadexG-25 gel, and carrying out rotary evaporation, thereby preparing the soybean-protein antibacterial agent. According to the method, the organic antibacterial agent is prepared from soybean protein; compared with the organic antibacterial agents, the bacteriostasis performance is increased by 2-5%, and the service time is prolonged by 20-30%; and compared with the inorganic antibacterial agents, the organic antibacterial agent is free of any heavy metal ion and is natural, pollution-free and environment-friendly.

Description

technical field [0001] The invention relates to a preparation method of a soybean protein antibacterial agent, belonging to the field of plastic additives. Background technique [0002] Bacteria and other pathogenic microorganisms are one of the main killers of human health. The surface pollution and breeding of various plastic products will pose a certain threat to the health of people who use and contact them. Adding a certain amount of antibacterial agents to these plastic products can kill bacteria or inhibit their reproduction within a certain period of time, keep themselves clean, and reduce the occurrence of people-to-people, people-to-things, and things-to-things caused by the use of these products. bacterial cross-contamination. Antibacterial agents are chemical components that are highly sensitive to some microorganisms such as bacteria, molds, fungi, and yeasts. They are added in a small amount in plastics, but they can play a role in sterilization while maintain...

Claims

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Application Information

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IPC IPC(8): A23L3/3526
CPCA23L3/3526Y02A40/90
Inventor 高大元高力群
Owner 高大元
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