Application of silver-coated palladium nano-preparation in the preparation of anti-cryptococcal infection drugs
A palladium nano- and cryptococcal technology, applied in the field of medicine, can solve the problems of no silver-coated palladium nano-preparation, no related reports on the combination of silver-coated palladium nano-preparation anti-cryptococcus drugs, and achieve good clinical application prospects, Improves the effect of antifungal action
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Embodiment 1
[0026] Example 1. Inhibition experiments of silver-coated palladium Pd@Ag nano-preparations with different diameters on a variety of common invasive fungal pathogens
[0027] Experimental Materials:
[0028] Synthetic raw materials required for silver-coated palladium nano-preparation: 2,4-palladium glutarate Pd (acac) 2 (99%) was purchased from AlfaAesar company, polyvinylpyrrolidone (PVP, MW=30000), dimethylformamide (DMF), sodium bromide (NaBr) and nitrate (AgNO 3 ) was purchased from Sinopharm Chemical Reagent Co., Ltd., and tetrabutylammonium bromide (TBAB) was purchased from Acros Organics. Fluconazole (FCZ) was purchased from Pfizer Pharmaceutical Co., Ltd., and the antifungal drug amphotericin B (AmB), RPMI 1640 liquid culture medium and PDA medium were all purchased from Sigma-Aldrich Company.
[0029] The standard strains of common pathogenic fungi used in the research were provided by the Shanghai Key Laboratory of Molecular Biology of Medical Fungi. Except for H9...
Embodiment 2
[0047] Example 2. Toxicity experiments of different concentrations of silver-coated palladium Pd@Ag nano-preparations on HeLa cells
[0048] Experimental Materials:
[0049] The silver-coated palladium Pd@Ag nano-preparation (5, 11, 30, 80, 120nm) is the same as that in Example 1. Human cervical cancer cells (HeLa) were purchased from the Institute of Cells, Chinese Academy of Sciences.
[0050] Reagents needed:
[0051] DMEM medium was purchased from Life Technologies, USA
[0052] CCK-8 kit was purchased from Shanghai Dongren Chemical Technology Co., Ltd.
[0053] equipment:
[0054] Synergy 2 multi-function detector (Germany BioTek product)
[0055] Carbon dioxide cell incubator (Japan Sanyo Electric Co., Ltd.)
[0056] SW-CT-IF ultra-clean workbench (Suzhou Antai Air Technology Co., Ltd.)
[0057] experimental method:
[0058] HeLa cells (1×10 4 cells / ml) in 96-well plate, DMEM medium, 37°C, 5% CO 2 Pre-culture for 24 hours in the cell culture incubator. After t...
Embodiment 3
[0062] Example 3. Bacteriostatic experiments of Pd@Ag nano-preparation (80nm) on clinical strains or isolates of different genotypes neoformans / Cryptococcus gattii
[0063] Experimental Materials:
[0064] The silver-coated palladium Pd@Ag nano-preparation (80nm) is the same as that in Example 1.
[0065] All Cryptococcus clinical or environmental isolates of different genotypes used in the study were provided by the Shanghai Key Laboratory of Molecular Biology of Medical Fungi, and their strain preservation numbers were SCZ9001-SCZ9045.
[0066] experimental method:
[0067] Combining the results of Example 1 and Example 2, we found that Pd@Ag nano-preparations with diameters of 11nm, 30nm, and 80nm all have good cryptococcal inhibition effect (MIC=0.5μg / mL), and have no obvious effect on HeLa cells at low concentrations. Toxicity and side effects, and compared with 11nm and 30nm nano-preparations, 80nm nano-preparations have a stronger antibacterial effect on other pathoge...
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