Application of TRPC6 in preparation of medicament for diagnosing and treating kidney ischemic reperfusion injury

A technology of renal ischemia reperfusion and medicine, applied in the field of biomedicine

Inactive Publication Date: 2015-12-23
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the mechanism of I / R occurrence and development has not been fully elucidated, there is currently no effective means of prevention and treatment of I / R clinically.

Method used

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  • Application of TRPC6 in preparation of medicament for diagnosing and treating kidney ischemic reperfusion injury
  • Application of TRPC6 in preparation of medicament for diagnosing and treating kidney ischemic reperfusion injury
  • Application of TRPC6 in preparation of medicament for diagnosing and treating kidney ischemic reperfusion injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Bioinformatics Analysis of the Molecular Mechanism of Renal Ischemia-Reperfusion Injury Based on Gene Chips

[0031] 1.1 Research materials

[0032] 1.1.1 Experimental animals

[0033] 6-week-old SD and BN rats weighing between 180-230 g were fed in the clean animal feeding room of the Experimental Animal Center of Southwest Hospital of Third Military Medical University. All rats were provided by the Experimental Animal Center of Daping Hospital, Third Military Medical University.

[0034] The animal room was kept clean, the indoor temperature was controlled by an air conditioner between 23°C and 28°C, the indoor humidity was controlled between 40% and 70%, and a 12h day and night cycle was maintained indoors. Animals had free access to food and water, and were observed daily and their body weight monitored.

[0035] 1.2 Research Methods

[0036] 1.2.1 Grouping of experimental animals

[0037] We selected GSE9943 in the NCBIGEO database, and there were 12...

Embodiment 2

[0054] Example 2 The role and mechanism of TRPC6 in regulating the programmed necrosis of human renal tubular epithelial cells in vitro ischemia-reperfusion injury

[0055] 2.1 Research materials

[0056] 2.1.1 Experimental cells

[0057] The human renal tubular epithelial cell line (HK-2) was preserved by the Kunming Cell Bank of the Chinese Academy of Sciences.

[0058] 2.2 Research Methods

[0059] 2.2.1 Immunofluorescence detection of TRPC6 expression on HK-2 cells

[0060] The HK-2 cells were subcultured into a special culture dish for laser confocal, and the old culture medium was discarded after continuing to culture for 24 hours. After washing with PBS, they were fixed with pre-cooled 4% paraformaldehyde at 4°C for 20 minutes. After washing with PBS for 3 times, 1% Triton-X100 was added to permeabilize for 10 min, and then washed with PBS for 3 times and then blocked with 1% BSA for 20 min. Then add rabbit anti-human TRPC6 polyclonal antibody diluted 1:50, overnigh...

Embodiment 3

[0146] Example 3 Experimental study on the protective effect and mechanism of TRPC6 regulating programmed necrosis on rat renal ischemia-reperfusion injury

[0147] 3.1 Research materials

[0148] 3.1.1 Experimental animals

[0149] SD rats are experimental animals described in Example 1.

[0150] 3.2 Research Methods

[0151] 3.2.1 Grouping of experimental animals

[0152] According to the principle of random block design, they were divided into: (1) blank control group (Control): without any treatment; (2) sham operation group (Sham): in the experiment, only the renal artery was separated after anesthesia to expose the renal pedicle without cutting The abdomen was closed after the renal artery was not clamped; (3) Ischemia-reperfusion group: the left kidney of the rat was excised after anesthesia, the right renal pedicle of the rat was separated, and the perfusion was restored after clamping with a non-destructive arterial clip for 40 minutes; (4) Ischemia-reperfusion+OA...

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Abstract

The invention relates to application of TRPC6 in preparation of a medicament for diagnosing and treating kidney ischemic reperfusion injury. The inventor researches the effect of the TRPC6 for regulating and controlling necroptosis in the kidney I/R (ischemic reperfusion) and a mechanism of the TRPC6 according to the relation between ion channel proteins and the kidney I/R injury, so that the effect of a signal passageway from TRPC6Ca to ions, then to CaMKII, and lastly to PARP-1 in the kidney ischemic reperfusion injury is achieved, a new idea and a new therapeutic target are provided for prevention and treatment of the kidney I/R, and the TRPC6 has an important practical application value.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the application of TRPC6 in the preparation of drugs for the diagnosis and treatment of renal ischemia-reperfusion injury. Background technique [0002] Ischemia-reperfusion injury (I / R) refers to the phenomenon that the degree of damage to tissues and organs is aggravated after the blood supply of tissues and organs is restored on the basis of ischemia. I / R is commonly found in the practice of various clinical departments, although a variety of I / R damage theories have been proposed, such as: abnormal oxygen, abnormal calcium, abnormal pH and so on. However, the complete pathogenesis of I / R has not been fully elucidated so far. Due to its unique physiological structure and functional characteristics, the kidney is a target organ that is highly susceptible to the stimulation of ischemia and hypoxia, so acute kidney injury (AKI) often occurs due to I / R. The typical pathological change...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/10A61P9/10A61P13/12
Inventor 申兵冰吴雄飞何跃
Owner THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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