Preparation methods for garbage enzyme and algal bloom inhibitor
A technology of environmentally friendly enzymes and inhibitors, applied in the direction of herbicides and algicides, botany equipment and methods, biochemical equipment and methods, etc., to help the recovery of the water body itself, inhibit the growth of algae, and contribute to purification Effect
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[0049] The invention also discloses a preparation method of environmental protection enzyme, which includes the following steps:
[0050] Put fruits and / or vegetables, water and sugars in a closed container in proportion to ferment for 3-6 months, and filter the filtered clear liquid to obtain the environmentally friendly enzyme.
[0051] Among them, the fermentation raw material may only contain fruits and not vegetables; for example, 1 / 3 apple peel, 1 / 3 banana peel and 1 / 3 orange peel are preferred. The fermentation raw material may also contain only vegetables and not fruits; for example, 1 / 3 lettuce, 1 / 3 cabbage and 1 / 3 water spinach are preferred.
[0052] Among them, the environmental protection enzyme is mixed and fermented according to the volume ratio of fruits and / or vegetables: water: sugar=1:2-4:8-12.
[0053] The invention also discloses a preparation method of the algal bloom inhibitor, which comprises the following steps:
[0054] The environmental protection enzyme prep...
Example Embodiment
[0064] Example 1 Inhibition of environmental protection enzymes on Microcystis aeruginosa
[0065] (1) Experimental supplies
[0066] Microcystis aeruginosa, enzymes;
[0067] Medium: sodium nitrate, dipotassium hydrogen phosphate, magnesium sulfate, calcium chloride, sodium carbonate, ferric citrate, Na 2 EDTA;
[0068] Ammonia-free water: concentrated sulfuric acid;
[0069] Total nitrogen: potassium nitrate, sodium hydroxide, potassium persulfate, concentrated hydrochloric acid;
[0070] Total phosphorus: ascorbic acid, ammonium molybdate, potassium antimony tartrate, potassium dihydrogen phosphate;
[0071] Ammonia nitrogen: ammonium chloride, potassium sodium tartrate, potassium iodide, mercury dichloride, potassium hydroxide;
[0072] Chemical oxygen demand: potassium dichromate, silver sulfate, ferrous sulfate, ferrous ammonium sulfate, 1,10-phenanthroline, mercury sulfate;
[0073] 25mL ground glass cuvette with stopper, 50mL ground glass cuvette with stopper, acid burette, 30mm cuv...
Example Embodiment
[0122] Example 2 Inhibition of environmental protection enzymes on the growth of Scenedesmus tetracera and improvement of water quality
[0123] Add the prepared environmentally friendly enzymes dropwise to the Scenedesmus tetracervus culture solution and set a blank control, three in parallel for each group.
[0124] On the 3rd day of the experiment, adding 1:240, 1:120 and 1:60 concentration of the algae broth cultured with environmentally friendly enzymes, it was obvious that Scenedesmus quadrifolium flocculated and precipitated to the bottom of the culture medium, and the higher the concentration of enzyme added in the culture medium , The smaller the amount of Scenedesmus quadrifolium flocculated. The water in the upper part of the algae culture medium with the environmental protection enzyme concentration of 1:240 becomes clear and transparent; the algae culture medium with the environmental protection enzyme concentration of 1:120 and 1:60 has the color of the enzyme itself ...
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