A kind of preparation method and application of aquatic product bacteriostatic agent

Abstract

The invention relates to a preparation method of an aquatic product antibacterial agent, which is characterized in that it comprises the following steps: dissolving an appropriate amount of carrageenan oligosaccharide in deionized water, adjusting the pH to 5.5-6.5, and adding carrageenan with a mass ratio of 1:2-4 A mixture of ferrous chloride and zinc chloride, wherein the mass ratio of carrageenan oligosaccharides to the mixture of ferrous chloride and zinc chloride is 5 to 8:1, and an appropriate amount of Vc is added for protection at the same time, and the reaction temperature is 25 to 30°C. The time is 2~3h; After the reaction finishes, after ethanol precipitation, centrifugation, the obtained carrageenan oligosaccharide-metal derivative precipitate, obtains carrageenan oligosaccharide-metal derivative after freeze-drying; The prepared carrageenan oligosaccharide Sugar-metal derivatives and carrageenan oligosaccharides are uniformly mixed at a ratio of 1 to 2.5:1 to obtain the desired antibacterial agent for aquatic products. The preparation process of the invention is simple, and the prepared bacteriostatic agent has remarkable bacteriostatic effect, can better bacteriostatically protect frozen aquatic products, is easy to clean and has no residue when eating, and can effectively ensure the edible quality of aquatic products while bacteriostatic and fresh. safety.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to a preparation method and application of an aquatic product antibacterial agent. Background technique [0002] Carrageenan (Carrageenan), also known as carrageenan, carrageenan, is an acidic polysaccharide extracted from red algae, with 1,4-D-galactopyranose and 1,3-D-galactose Skeleton structure of pyranose (or 3,6-endo-D-galactopyranose) disaccharide unit. Studies have found that carrageenan has various biological activities such as lowering blood lipids, anticoagulation, antithrombotic, immune regulation, and stimulating connective tissue growth. However, due to the large molecular weight of carrageenan, its solubility and absorbability are poor, which limits its application. . [0003] Carrageenan oligosaccharides are obtained by degrading carrageenan with carrageenan-degrading enzymes. Compared with carrageenan, carrageenan oligosaccharides have smaller molecular weight...

AI Technical Summary

Problems solved by technology

Studies have found that carrageenan has various biological activities such as lowering blood lipids, anticoagulation, antithrombotic, immune regulation, and stimulating connective tissue growth. However, due to the large molecular weight of carrageenan, its solubility and absorbability are poor, which limits its application.