The invention discloses an acid soil improvement fertilizer and a preparation method thereof. The acid soil improvement fertilizer is composed of improvement plant ash, special bacillus subtilis agent, shell calcium powder, high-molecular improving anionic polyacrylamide and microelements containing borax and zinc sulfate. The preparation comprises thoroughly and uniformly mixing the materials above in a mixer and performing physical granulation through an extrusion device. According to the practical situation and through long-term practice research and pilot plant test, the acid soil improvement fertilizer is proved to take effects 1-3 months after application, be capable of improve acid soil, loosen soil, improve soil fertility, improve soil ph and have no damage to other soil properties. Meanwhile, the acid soil improvement fertilizer can rapidly supplement organic matters, nutritious elements such as carbon, calcium, silicon, magnesium and zinc and microbial flora, improve soil permeability and activity of microorganisms, fundamentally improve the microbial chain of soil, reduce volume weight of soil, enhance soil porosity and improve the content of soil organic matters and the activity of the microbial flora. Besides, the acid soil improvement fertilizer is low in cost and significant in effect during production application.