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Preparation method of epicatechin monomer

A technology of epicatechin and green tea catechin, which is applied in the field of preparation of epicatechin monomers, can solve the problems of no epicatechin, etc., and achieve the effect of short preparation period, low cost and anti-aging function

Inactive Publication Date: 2016-01-06
HUBEI ZHONGXIN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the preparation of epicatechin (EC) monomer at home and abroad

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] 1. Take 100 grams of large-leaf green tea catechin extract, make it into 2000ml catechin solution, add citric acid to adjust the pH to 4.0-5.0, add 0.5 g of tannase to the solution, and heat it at 30-35°C Enzymolysis for 4 hours, stirring for 5 minutes at intervals of 30 minutes, and centrifugal filtration of the enzymolysis solution at 1000 r / min;

[0017] 2. Slowly pass the filtered supernatant in step 1 through the chromatographic column equipped with ADS-8 adsorption resin, and use 10%, 30%, and 80% ethanol aqueous solution to elute sequentially, the amount of adsorption resin: 50 times Large-leaf green tea catechin extract weight; Upper column flow rate: 1.0BV / h; The consumption of three kinds of ethanol aqueous solutions of different concentrations is 2BV; Desorption flow rate: 1.0BV / h; Collect 30% ethanol eluate, and Concentrating the eluent to 16-18 degrees Baume to obtain a concentrated solution;

[0018] 3. Place the concentrated solution at a temperature of ...

Embodiment 2

[0020] 1. Take 100 grams of large-leaf green tea catechin extract, make it into 2000ml catechin solution, add citric acid to adjust the pH to 4.0-5.0, add 0.5 g of tannase to the solution, and heat it at 30-35°C Enzymolysis was performed for 5 hours, with intermittent stirring for 30 minutes and 5 minutes, and the enzymolysis solution was centrifuged and filtered at 1000 r / min.

[0021] 2. Slowly pass the filtered supernatant in step 1 through the chromatographic column equipped with HPD-450 adsorption resin, and elute with 10%, 30%, and 80% ethanol aqueous solution respectively. The amount of adsorption resin: 50 times larger Leaf green tea catechin extract weight; Upper column flow rate: 1.0BV / h; The consumption of the ethanol aqueous solution of three kinds of different concentrations is 2BV; Desorption flow rate: 1.0BV / h; Collect 30% ethanol eluate, and The eluent is concentrated to 16-18 Baume degrees to obtain a concentrated solution.

[0022] 3. Place the concentrated ...

Embodiment 3

[0024] 1. Take 100 grams of large-leaf green tea catechin extract, make it into 2000ml catechin solution, add citric acid to adjust the pH to 4.0-5.0, add 0.5 g of tannase to the solution, and heat it at 30-35°C Enzymolysis was performed for 6 hours, with intermittent stirring for 30 minutes and 5 minutes, and the enzymolysis solution was centrifuged and filtered at 1000 r / min.

[0025] 2. Slowly pass the filtered supernatant in step 1 through the chromatographic column equipped with HPD-450A adsorption resin, and elute with 10%, 30%, and 80% ethanol aqueous solution respectively. The amount of adsorption resin: 50 times larger Leaf green tea catechin extract weight; Upper column flow rate: 1.0BV / h; The consumption of the ethanol aqueous solution of three kinds of different concentrations is 2BV; Desorption flow rate: 1.0BV / h; Collect 30% ethanol eluate, and The eluate is concentrated to 16-18 degrees Baume to obtain a concentrated solution;

[0026] 3. Place the concentrated...

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Abstract

The invention relates to a preparation method of an epicatechin monomer. The preparation method comprises the following steps of: (1) adding tannase to a solution containing large-leaf green tea catechin extract, carrying out static enzymolysis at 30-35 DEG C, intermittently stirring, and centrifugally filtering to obtain an enzymolysis solution; (2) introducing the enzymolysis solution into a chromatographic column loaded with adsorptive resin, carrying out gradient elution with ethanol elution agent, collecting eluate, concentrating eluate to 16-18 Baume degree to obtain concentrate, standing the concentrate at 0-4 DEG C for 48-72h, adding a little of epicatechin monomer, crystallizing and recrystallizing, centrifuging and drying to obtain the epicatechinmonomer. According to the method, epicatechin gallate is subjected to enzymolysis by using tannase, so that the method has good transformation effect, high transformation rate and high product quality, short preparation period, and low cost, and is suitable for scaled production.

Description

technical field [0001] The invention belongs to the field of preparation of catechins, and in particular relates to a preparation method of epicatechin monomers. Background technique [0002] Catechin is the most important physiologically active substance in tea, accounting for about 80% of polyphenols, belonging to flavanols, and its basic structure is α-phenylbenzopyran, which is divided into two types: ester type and non-ester type , the main difference in structure lies in the number and position of hydrogen substitution. Catechin mainly consists of epigallocatechin gallate (EGCG), epigallocatechin (EGC), epicatechin gallate (ECG) and epicatechin (EC) monomers. Catechin has obvious pharmacological functions such as anti-aging, anti-cancer and anti-mutation, reducing fat and losing weight, lowering blood sugar, blood lipid and cholesterol, and preventing cardiovascular diseases. It has been widely used in food processing, medical care, and daily chemical industry. The M...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/06C07D311/62
Inventor 叶艳龙菲菲罗祥马松吴世华
Owner HUBEI ZHONGXIN BIOTECH CO LTD
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