Method for preparing trichophyton sample for scanning electron microscope

Abstract

The invention provides a method for preparing a trichophyton sample for a scanning electron microscope. The method is mainly characterized in that when the sample is fixed, ultrasonic wave aided treatment is applied during application of treatment with a glutaraldehyde solution. Instead of application of virulent osmic acid, ultrasonic wave aided treatment with glutaraldehyde is innovatively applied, and the new method is provided for preparing the trichophyton sample for the scanning electron microscope and has the advantages of excellent fixing effects, simplicity and convenience in operation, safety and low toxicity.

Description

technical field [0001] The invention relates to a preparation method of a trichophyton scanning electron microscope sample, belonging to the field of biotechnology. Background technique [0002] The pretreatment of samples is an essential process for electron microscope observation, and the quality of the treatment effect is directly related to the results of electron microscope observation. [0003] Since the hyphae of Trichophyton are lumpy and some are thick, there is a phenomenon of insufficient contact with the reagent during the sample processing process, resulting in uneven sample processing, and the results cannot be accurately judged. Through many repeated experiments, it is found that the most important step in the electron microscope sample preparation process is fixation. If the fixation effect is not good, it is difficult to improve the follow-up treatment. [0004] The most commonly used method is glutaraldehyde-osmic acid (osmium tetroxide) double immobilizat...

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Problems solved by technology

[0003] Since the hyphae of Trichophyton are lumpy and some are thick, there is a phenomenon of insufficient contact with the reagent during the sample processing process, resulting in uneven sample processing, and the results cannot be accurately judged

Through repeated experiments, it is found that the most important step in the electron microscope sample preparation process is fixation. If the fixation effect is not good, it is difficult to improve the follow-up treatment.

[0004] The most commonly used method is glutaraldehyde-osmic acid (osmium tetroxide) double immobilization at present, but osmic acid is highly toxic, and contact or inhalation can cause headache, respiratory tract inflammation, eye disease, bronchial disease, pneumonia, etc. Health poses a serious potential threat, and it is necessary to study a low-toxicity fixation method to replace the traditional double fixation method

There are also studies using glutaraldehyde alone for fixation, but it is easy to shrink the bacteria and it is difficult to achieve satisfactory results.

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