Preparation method for polymorphic bio-polypeptide organic fertilizer

A biological polypeptide and organic fertilizer technology, which is applied in the preparation of organic fertilizers and multi-morphic biological polypeptide organic fertilizers, can solve the problems of unfavorable large-scale production of organic fertilizers, single polypeptide forms, and limited biological activity, and achieve rich polypeptide forms, Increased Vc content and good fertilizer effect

Active Publication Date: 2016-01-20
德州八虎生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although acid hydrolyzed protein can also produce polypeptides, the obtained polypeptides have a single form and limited biological activity. Meanwhile, CN101139221A only uses castor cake (meal), rapeseed cake (meal), bean cake (meal) as raw mater

Method used

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  • Preparation method for polymorphic bio-polypeptide organic fertilizer

Examples

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Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: a kind of preparation method of polymorphic biological polypeptide organic fertilizer, the steps are as follows:

[0032] (1) Take 350 parts of crushed peanut stalks, 320 parts of culture material waste residues for cultivating Pleurotus eryngii, put them in a container, add 900 parts of water, mix well, and place them at 25°C for 5 days; then take 180 parts of wheat bran , add 150 parts of soybean meal, and place it at 28°C for another 40 hours. After the end, pass in water vapor, keep it for 25 minutes when it reaches 95°C, and then drop it to 40-45°C to obtain a mixture and set it aside;

[0033] (2) Take 130 parts of the mixture prepared in step (1), adjust the pH to 8.0, add 0.4 parts of alkaline protease preparation, mix well, and place it at 45°C for 6 hours. During this period, stir once every 1 hour to obtain Protease breakdown product Ⅰ;

[0034] (3) Adjust the pH of the protease decomposition product I prepared in step (2) to 7.0, add 0.3 parts...

Embodiment 2

[0037] Embodiment 2: a kind of preparation method of polymorphic biological polypeptide organic fertilizer, the steps are as follows:

[0038] (1) Take 300 parts of crushed peanut stalks, 450 parts of culture material waste residue for cultivating Pleurotus eryngii, put them in a container, add 800 parts of water, mix well, and place them at 28°C for 4 days; then take 130 parts of wheat bran , add 120 parts of soybean meal, and place it at 25°C for another 40 hours. After the end, pass in water vapor, keep it for 30 minutes when it reaches 95°C, and then drop it to 40-45°C to obtain a mixture and set it aside;

[0039] (2) Take 150 parts of the mixture prepared in step (1), adjust the pH to 8.2, add 0.5 parts of alkaline protease preparation, mix well, and place it at 50°C for 5 hours. During this period, stir once every 1 hour to obtain Protease breakdown product Ⅰ;

[0040] (3) Adjust the pH of the protease decomposition product I prepared in step (2) to 7.2, add 0.4 parts ...

Embodiment 3

[0043] Embodiment 3: a kind of preparation method of polymorphic biological polypeptide organic fertilizer, the steps are as follows:

[0044] (1) Get 420 parts of crushed peanut stalks, 350 parts of culture material waste residues for cultivating Pleurotus ostreatus, put them in a container, add 1000 parts of water, mix well, and place them for 7 days at 22°C; then take 100 parts of wheat bran, Add 130 parts of soybean meal, and place it at 23°C for another 48 hours. After the end, pass in water vapor, keep it for 20 minutes when it reaches 95°C, and lower it to 40-45°C to obtain a mixture for use;

[0045] (2) Take 100 parts of the mixture prepared in step (1), adjust the pH to 7.8, add 0.3 parts of alkaline protease preparation, mix well, and place it at 48°C for 4 hours. During this period, stir once every 1 hour to obtain Protease breakdown product Ⅰ;

[0046] (3) Adjust the pH of the protease decomposition product I prepared in step (2) to 6.8, add 0.3 parts of neutral ...

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Abstract

The invention relates to a preparation method for a polymorphic bio-polypeptide organic fertilizer. The preparation method comprises the following steps: with high-protein content peanut straw, domestic fungus culture medium waste residue, wheat bran and soybean meal as raw materials, carrying out water extraction so as to extract protein in the raw materials; and successively adding an alkali protease preparation, a neutral protease preparation and an acidic protease preparation and carrying out three-level decomposition so as to prepare the polymorphic bio-polypeptide organic fertilizer. The preparation method does no need complex and energy-consuming after-treatment procedures like separation and purification, so production cost of the polypeptide organic fertilizer is substantially reduced; and since the polypeptide organic fertilizer contains polymorphic polypeptides, the fertilizer has improved fertilizer efficiency, and field test results show that the fertilizer has good yield increasing effect.

Description

technical field [0001] The invention relates to a method for preparing an organic fertilizer, in particular to a method for preparing a polymorphic biological polypeptide organic fertilizer, and belongs to the technical field of organic fertilizer production. Background technique [0002] Polypeptides refer to small molecular active substances with the same structure and composition as proteins, generally consisting of 2-100 amino acids. Exogenous polypeptides can not only provide crops with organic nutrients, but also regulate the physiological functions of crops, activate the activity of crop cells, improve the stress resistance of crops, and reduce the incidence of crop disease. In recent years, there have been more and more studies on the use of polypeptides in crop cultivation to save fertilizers and improve fertilizer utilization, and more and more attention has been paid to fertilizers containing polypeptides. For example, CN103204735A (CN201310116458) discloses a wat...

Claims

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Application Information

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IPC IPC(8): C05F11/10C05F15/00
Inventor 辛寒晓王泽利赵玉宝李宏云宋渊博
Owner 德州八虎生物科技有限公司
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