Check patentability & draft patents in minutes with Patsnap Eureka AI!

Method for establishing salinispora arenlocola CNS-205 gene genetic manipulation system

A marine actinomycete and operating system technology, which is applied in the field of construction of marine actinomycete gene genetic operating system, and can solve the problem of no construction method of marine actinomycete gene genetic operating system.

Inactive Publication Date: 2016-01-20
FOSHAN UNIVERSITY
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the above-mentioned deficiencies in the prior art, the purpose of the present invention is to provide a method for constructing a genetic operating system for marine actinomycetes, aiming to solve the problem that there is no method for constructing a genetic operating system for marine actinomycetes in the prior art. question

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for establishing salinispora arenlocola CNS-205 gene genetic manipulation system
  • Method for establishing salinispora arenlocola CNS-205 gene genetic manipulation system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0033] Experimental Materials

[0034] Gene clone recipient E.coliDH10B, conjugative transfer donor strain E.coliET12567 (pUZ8002), integrated plasmids pSET152 and pIB139 (both carrying apramycin resistance gene aac(3)IV, conjugative transfer site oriT).

[0035] Reagent

[0036] Thiostrepton and apramycin were purchased from Sigma, USA; chloramphenicol, ampicillin, kanamycin, thiostrepton, Lambda / Hind, restriction endonuclease, DNA recovery kit, Plasmid extraction kits were purchased from Dalian Bao Biological Engineering Co., Ltd.; other commonly used reagents were purchased from Shanghai Sinopharm Group.

[0037] Media and Antibiotics

[0038] The plate medium of SalinisporaarenlocolaCNS-205 is SFMS, the culture medium of bacteria is LB medium, the medium of spore pre-germination is 2×YT, and the medium of conjugative transfer is SFMS medium.

[0039] The antibiotic concentration in LB was: apramycin 25 μg / mL, chloramphenicol 25 μg / mL, kanamycin 25 μg / mL, ampicillin 100 μg...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for establishing a salinispora arenlocola CNS-205 gene genetic manipulation system. The method includes the steps that a sterilized SFMS culture medium is melted, the temperature is lowered to be 40-50 DEG C, an appropriate amount of antibiotic is added in the SFMS culture medium, a flat plate is inverted, salinispora arenlocola CNS-205 spore suspension coats the flat plate, and culture is conducted for 4-5 days at the temperature of 25-30 DEG C; the unified plasmid is introduced into a conjugational transfer donor through a CaCl2 method, and the unified plasmid is introduced into a salinispora arenlocola CNS-205 spore obtained in the step A from the donor in a conjugational transfer mode to be integrated by the aid of tra genes on the donor. According to the technical scheme, the probability of introducing exogenous DNA fragments into the salinispora arenlocola CNS-205 is realized.

Description

technical field [0001] The invention relates to the technical field of biological genetic engineering, in particular to a method for constructing a marine actinomycetes genetic genetic operating system. Background technique [0002] Streptomyces (Streptomyces) belongs to Prokaryote Kingdom, Actinomycetes, and Streptomycetaceae in taxonomy. It is a kind of aerobic Gram-positive bacteria with branched filaments and is one of the main microbial groups in soil. . The average genome size of Streptomyces is 8000kb, about twice that of E. coli genome. Compared with other organisms, one of the biggest characteristics of its genome is that its DNA has a very high G+Cmol%, which can be as high as 69~78%, which is one of the biological groups with the highest G+Cmol% content known so far. Streptomyces are one of the most commercially valuable groups of industrial microorganisms. It has been known that nearly 70% of antibiotics in nature are produced by Streptomyces and their close r...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/76
Inventor 马艳玲陈婉容刑福炜李锐
Owner FOSHAN UNIVERSITY
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com