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Method of preparing butanol from a butyric acid-containing aqueous fermentative liquid

A fermentation broth and butyric acid technology, which is applied in the preparation of organic compounds, carboxylate salts, carboxylate esters, etc., can solve the problems of high acid and alkali, unsatisfactory, etc.

Inactive Publication Date: 2016-01-27
GREEN CELLULOSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Through the aforementioned method, although high-purity butyric acid can be produced, a high amount of acid and alkali is required (1 mole of sodium hydroxide and 1 mole of hydrochloric acid are required to prepare 1 mole of butyric acid), so it is not ideal

Method used

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  • Method of preparing butanol from a butyric acid-containing aqueous fermentative liquid
  • Method of preparing butanol from a butyric acid-containing aqueous fermentative liquid
  • Method of preparing butanol from a butyric acid-containing aqueous fermentative liquid

Examples

Experimental program
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Effect test

preparation example

[0059] Preparation Example: Preparation of Aqueous Fermentation Broth Containing Butyric Acid

[0060] [Microorganism culture]

[0061] In this preparation example, the microorganism Clostridium tyrobutylicum (Clostridium tyrobutylicum, ATCC25755) used was purchased from the Bioresource Collection and Research Center (BCRC). Before use, the stock culture was anaerobically pre-cultured (pre-culture) with a serum bottle containing 100 ml of reinforced Clostridium medium (Reinforced Clostridial Medium, RCM; purchased from Merck) at 37°C with stirring. 48 hours.

[0062] Prepare the basal medium (basalmedium), which contains the following components per liter of deionized water: 5 grams of yeast extract (yeastextract), 5 grams of peptone (peptone), 3 grams of ammonium sulfate, 1.5 grams of potassium dihydrogen phosphate, 0.6 grams of hydrous magnesium sulfate (MgSO 4 ·7H 2 O) and 0.03 g of hydrous ferric sulfate (FeSO 4 ·7H 2 O) (see Wu et al., Biotechnology and Bioengineeri...

Embodiment 1

[0068] Embodiment 1: obtain butyric acid by extracting in the fermented liquid

[0069] [extraction with trioctylamine]

[0070] 18,941 grams of fermentation broth were provided, which had a pH of 6.6 and contained 2.4% butyric acid by weight. After first adjusting the pH value of the fermentation broth to about 2.43 with sulfuric acid, as figure 2 As shown, the fermentation broth was divided into five batches with weights of 4,000, 3,675, 4,000, 3,500, and 3,766 grams in sequence, and each batch of fermentation broth was subjected to secondary cross-flow extraction with trioctylamine. In stage 1, 500 grams of trioctylamine were mixed with the fermentation broth and extracted while maintaining the pH of the aqueous phase at about 1.28 with sulfuric acid. In stage 2, 487 grams of trioctylamine was mixed with the stage 1 raffinate (ie, the aqueous phase) and extracted, maintaining the pH of the aqueous phase at about 2.58 with sulfuric acid. The extracts of each stage (ie, t...

Embodiment 2 to 4

[0075] Examples 2 to 4: Esterification

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Abstract

A method of preparing butanol from a butyric acid-containing aqueous fermentation broth is disclosed, wherein the butyric acid-containing aqueous fermentation broth is obtained from the fermentation of a substrate with the use of a microorganism. The method includes the steps as follows: a) mixing an extracting solvent containing water-insoluble trialkylamine with an aqueous fermentation broth and performing liquid / liquid extraction so as to obtain an aqueous phase and an non-aqueous phase containing trialkyl ammonium butyrate; b) heating the non-aqueous phase obtained at the step a) and decomposing the trialkyl ammonium butyrate so as to obtain butyrate and trialkyl ammonium; c) performing esterification reaction of the butyrate acquired at the step b) and carbinol so as to obtain methyl butyrate; and d) performing hydrogenolysis reaction for the methyl butyrate acquired at the step c) so as to obtain butanol and carbinol.

Description

technical field [0001] The present invention relates to a method for producing biomass butanol, in particular to a method for preparing butanol from an aqueous fermentation broth containing butyric acid, wherein the aqueous fermentation broth containing butyric acid is produced by using microorganisms on carbohydrates obtained by fermentation. Background technique [0002] In today's limited resources, due to the dwindling oil resources, in order to solve the energy problem, there has been research on the production of bioethanol as fuel. The so-called "bio-ethanol" refers to ethanol produced by fermentation of sugars in crops by microorganisms, which can be used alone or mixed with gasoline as fuel. [0003] However, using ethanol as fuel is not without its disadvantages. One of the known disadvantages is that there is usually more or less water vapor in the environment. In gasoline, ethanol is miscible with water and separated from gasoline, which is not conducive to the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C29/149C07C31/12
CPCC07C29/149C07C67/08C07C31/12C07C69/24C07C51/02C07C51/412C07C51/48C12P7/52C07C53/124
Inventor 童迁祥施瑞虎林昀辉李宏台
Owner GREEN CELLULOSITY