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Preparation method of gold nanoflowers for targeting therapy of tumors

A gold nanoflower, tumor targeting technology, applied in the field of nano-biomedical materials, can solve problems such as unfavorable control of the size and distribution of gold nanoflowers, and achieves production and application, high biological safety, and strong operability. Effect

Active Publication Date: 2016-02-03
江苏中方基因生物医学科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this post-polymer modification method is not conducive to controlling the size and distribution of gold nanoflowers.

Method used

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  • Preparation method of gold nanoflowers for targeting therapy of tumors
  • Preparation method of gold nanoflowers for targeting therapy of tumors
  • Preparation method of gold nanoflowers for targeting therapy of tumors

Examples

Experimental program
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Effect test

Embodiment 1

[0027] A certain amount of HAuCl 4 Dissolve in 10mL water to make 0.5mM HAuCl 4 aqueous solution. After dissolving the polythiol hydrophilic polymer in water to make a 1mM solution, add it dropwise to HAuCl 4 in the aqueous solution. Then, folic acid (1 mM), a tumor-targeting molecule with succinimide-modified ends, was added to the above solution. After stirring for 30 minutes, 5 mM ascorbic acid was added. After the reactants were reduced, a light blue nano gold flower solution a was obtained.

[0028] Add 0.5 mL of hydrazine hydrate to 5 mL of the above light blue golden flower solution a to reduce the methyl ester group at the end of the hydrophilic polymer to hydrazide. Centrifuge the reacted solution three times at 800 rpm, remove the supernatant, redisperse the nano-golden flower in dimethyl sulfoxide (DMSO), add 5 mg of anti-tumor drug doxorubicin, and dissolve completely Then, stir at room temperature for 24 hours. The anti-tumor drug doxorubicin is linked to t...

Embodiment 2

[0030] A certain amount of HAuCl 4 Dissolve in 10mL water to make 0.5mM HAuCl 4 aqueous solution. After dissolving the polythiol hydrophilic polymer in water to make a 2mM solution, add it dropwise to HAuCl 4 in the aqueous solution. Then, the tumor-targeting molecule polypeptide RGD (0.1 mM) whose terminal was modified with succinimide was added to the above solution. After stirring for 30 minutes, 10 mM ascorbic acid was added. After the reactants were reduced, a light blue nano gold flower solution a was obtained.

[0031] Add 0.5 mL of hydrazine hydrate to 5 mL of the above light blue golden flower solution a to reduce the methyl ester group at the end of the hydrophilic polymer to hydrazide. Centrifuge the reacted solution three times at 800 rpm, remove the supernatant, redisperse the nano-golden flower in dimethyl sulfoxide (DMSO), add 10 mg of anti-tumor drug doxorubicin, and dissolve completely Then, stir at room temperature for 24 hours. The anti-tumor drug dox...

Embodiment 3

[0033] A certain amount of HAuCl 4 Dissolve in 10mL water to make 0.5mM HAuCl 4 aqueous solution. After dissolving the polythiol hydrophilic polymer in water to make a 5mM solution, add it dropwise to HAuCl 4in the aqueous solution. Then, tumor-targeting molecular aptamers (3 mM) with succinimide-modified ends were added to the above solution. After stirring for 30 minutes, 20 mM ascorbic acid was added. After the reactants were reduced, a light blue nano gold flower solution a was obtained.

[0034] Add 0.5 mL of hydrazine hydrate to 5 mL of the above light blue golden flower solution a to reduce the methyl ester group at the end of the hydrophilic polymer to hydrazide. Centrifuge the reacted solution three times at 800 rpm, remove the supernatant, redisperse the nano-golden flower in dimethyl sulfoxide (DMSO), add 5 mg of anti-tumor drug doxorubicin, and dissolve completely Then, stir at room temperature for 24 hours. The anti-tumor drug doxorubicin is linked to the s...

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Abstract

The invention relates to a preparation method of gold nanoflowers for targeting therapy of tumors. According to the method, by controlling various reaction conditions to prepare the gold nanoflowers differing in dimension, modifying a hydrophilic polymer in situ on the surface through a mercapto group, selectively linking different tumor-targeting molecules on the hydrophilic polymer through a reaction between the mercapto group and succinimide and linking antineoplastic drugs of different numbers onto the surfaces of the gold nanoflowers by stimulating a responsive acylhydrazone bond, the gold nanoflowers which have the property of tumor targeting and combine photo-thermal therapy and chemotherapy are prepared. The gold nanoflowers have the characteristics of near-infrared responsiveness, good stability, good biocompatibility and the like. An obtained finished product can meet demands of clinical application.

Description

technical field [0001] The present invention relates to a preparation method of gold nanoflowers for tumor targeting therapy, in particular to a preparation technology for in-situ modification of gold nanoflowers by polymercapto polymers, simultaneously loaded with tumor targeting molecules and anti-tumor drugs, used for Tumor targeted therapy combined with photothermal therapy and chemotherapy. The invention belongs to the field of nano biomedical materials. Background technique [0002] In recent years, malignant tumor (cancer) has become one of the major diseases that endanger human health. The treatment of cancer has become a hot spot in modern medical research. At present, the clinical treatment of cancer mainly relies on radiotherapy, chemotherapy and surgery. These methods alleviate the suffering of patients to a certain extent, but they also have certain limitations, which make the final cancer cure rate of patients lower. The continuous integration of nanotechnol...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K47/48A61K31/704A61P35/00
Inventor 何丹农庄园园金彩虹
Owner 江苏中方基因生物医学科技有限公司
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