Live vaccine heat-resistant protective agent and its preparation method and application

Abstract

The invention discloses a heat-resisting protective agent for a live vaccine, and a preparation method and application of the heat-resisting protective agent, and belongs to the field of preparation and application of a heat-resisting protective agent for a live vaccine. The agent comprises components of trehalose, glucose, gelatin, thiourea, and polyvinylpyrrolidone K30. An avian infectious bronchitis live vaccine prepared through application of the heat-resisting protective agent is short in freeze-drying time and low in cost; decrease of titer of the vaccine is small; and the efficacy of the vaccine maintains stable. According to the invention, a manner of freeze-drying of the avian infectious bronchitis live vaccine prepared from the heat-resisting protective agent is further optimized; the freeze-drying time is shortened from 48h to 24h; and the decrease of titer of the vaccine after heating is small. The vaccine reaches can be stored for 36 months at 2-8 DEG C, and is relatively stable in efficacy and good in safety.

Description

technical field [0001] The invention relates to a heat-resistant protective agent for live vaccines, and also relates to a preparation method and application of the heat-resistant protective agent, belonging to the field of preparation and application of the heat-resistant protective agent for live vaccines. Background technique [0002] Chicken infectious bronchitis (IB) is an acute highly contagious disease caused by chicken infectious bronchitis virus (IBV), manifested as symptoms such as coughing, sneezing, and tracheal rales. Chicken infectious bronchitis has caused serious economic losses to the world poultry industry. [0003] China's research on heat-resistant lyoprotectants started relatively late, but research in this area has achieved certain results. The material components of existing heat-resistant lyoprotectants are usually: gelatin, bovine serum albumin, Sucrose, PVPK30, sorbitol, sodium glutamate, Vc, when used, the high molecular compound should be sterili...

AI Technical Summary

Problems solved by technology

After the freeze-dried vaccine was stored at 2-8°C for 24 months, the virus titer did not decrease significantly, and it was placed at 37°C for 10 days, and the EID 50 The titer drops by about 0.8, and the whole process of freeze-drying takes about 48 hours; there are disadvantages such as complex formula and preparation process, long freeze-drying time, and much lower virus content after heat resistance

[0004] Therefore, it will be of great significance to improve the formula and freeze-drying curve of the existing protective agent, and solve the problems of complex preparation procedures, long freeze-drying time, large drop in vaccine titer after heat resistance, and high cost of the existing heat-resistant protective agent.

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