Preparation method of high purity sulforaphene

A kind of rapin, high-purity technology, applied in the field of preparation of high-purity rapin, to achieve the effect of saving cost, improving stability and high purification efficiency

Active Publication Date: 2016-02-03
BEIKE HUAXIA BIOMEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a kind of preparation method of high-purity radishine aiming at the deficiencies of existing methods for producing radishine. The method has the advantages of continuous, simple and stable production process, high extraction and purification efficiency, high yield and comprehensive resources. High utilization rate, no harmful substance discharge, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A kind of preparation method of high-purity radashin, concrete method step is as follows:

[0026] (1) Preparation of radish concentrate

[0027] Taking radish seed meal as raw material, according to the mass (kg) of radish seed meal: the volume fraction is 50~80% ethanol solution volume (L) ratio is the ratio of 1: 6, first stirred and reacted at room temperature for 4h, and then used The citric acid solution with a mass fraction of 2% was adjusted to pH 2, and then pumped into a clarified tubular centrifuge, centrifuged at a speed of 15000 r / min, and the supernatant and sediment were collected respectively. For the centrifugal supernatant that collects, pump into the reverse osmosis concentrator, under 0.15MPa, carry out the reverse osmosis concentration for the first time, until the volume of the first reverse osmosis retentate is reduced to 10% of the original volume, collect the second Primary reverse osmosis permeate and primary reverse osmosis retentate. In the f...

Embodiment 2

[0036] A kind of preparation method of high-purity radashin, concrete method step is as follows:

[0037] (1) Preparation of radish concentrate

[0038] Taking radish seed meal as raw material, according to the mass (kg) of radish seed meal: volume fraction is the ratio of the volume (L) of 65% ethanol solution to be the ratio of 1:7, stir and react at room temperature for 5h first, then use mass fraction The pH of the 4% citric acid solution was adjusted to 2.5, centrifuged at a rotational speed of 18000 r / min, and the supernatant and sediment were collected respectively. For the centrifugal supernatant that collects, pump in the reverse osmosis concentrator, under 0.20MPa, carry out reverse osmosis concentration for the first time, until the volume of reverse osmosis retentate for the first time is reduced to 12% of original volume, collect respectively Primary reverse osmosis permeate and primary reverse osmosis retentate. In the first reverse osmosis retentate collected,...

Embodiment 3

[0047] A kind of preparation method of high-purity radashin, concrete method step is as follows:

[0048] (1) Preparation of radish concentrate

[0049] Taking radish seed meal as raw material, according to the mass (kg) of radish seed meal: volume fraction is the ratio of the volume (L) of 80% ethanol solution to be the ratio of 1:9, stir and react at room temperature for 7h first, and then use mass fraction The pH of the 6% citric acid solution was adjusted to 3, centrifuged at a speed of 20000 r / min, and the supernatant and sediment were collected respectively. For the centrifugal supernatant that collects, pump in the reverse osmosis concentrator, under 0.25MPa, carry out reverse osmosis concentration for the first time, until the volume of the reverse osmosis retentate for the first time is reduced to 15% of original volume, collect respectively Primary reverse osmosis permeate and primary reverse osmosis retentate. In the first reverse osmosis retentate collected, add ...

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Abstract

The invention discloses a preparation method of high purity sulforaphene and belongs to the field of sulforaphene preparation. High purity sulforaphene is prepared from radish seed meal as a raw material by ethanol extraction, enzyme film reaction, chromatogram preparation and vacuum thin film evaporation. The preparation method has the characteristics of continuous production technology, simpleness, stability, high extraction and purification efficiency, high yield, high resource comprehensive utilization rate and no harmful substance discharge. In production, two enzymes are used as catalysts so that a sulforaphene enzymolysis yield is greatly improved. The preparation method of high purity sulforaphene has high purifying efficiency, realizes product purity of 98% or more and does not produce solvent residues. The high purity sulforaphene can be widely used in industries of medicines and health products.

Description

1. Technical field [0001] The invention belongs to the technical field of producing radishine, in particular to a preparation method of high-purity radishine. 2. Technical background [0002] Glucosinolates (glucosinolates for short) are important secondary metabolites of cruciferous plants. Glucosinolates and their degradation products play an important role in plant resistance to pests and diseases, special flavor composition and human health. Radishes also contain glucosinolates, but the types of glucosinolates in radishes are different from those in other cruciferous plants. The main glucosinolates in radishes are 4-methylsulfinyl-3-butenyl glucosinolates (commonly known as glucoraphenin, glucoraphenin). The content of radish is the highest in radish seeds, and it can be degraded by myrosinase to generate 4-methylsulfinyl-3-butenyl isothiocyanate (commonly known as sulforaphene). Studies have shown that radishin has extremely high biological activity in inhibiting cell ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C331/22
Inventor 张杰余春阳王星敏刘帆邵承斌
Owner BEIKE HUAXIA BIOMEDICAL TECH CO LTD
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