A kind of preparation method of high-purity rataxin

A kind of rapin, high-purity technology, applied in the field of preparation of high-purity rapin, to achieve the effects of improving stability, good stability and simple production equipment

Active Publication Date: 2017-04-05
BEIKE HUAXIA BIOMEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The object of the present invention is to provide a kind of preparation method of high-purity radishine aiming at the deficiencies of existing methods for producing radishine, which has the advantages of continuous, simple and stable production process, high extraction and purification efficiency, high yield and comprehensive resources High utilization rate, no emission of harmful substances, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A method for preparing high-purity lysine, the specific steps are as follows:

[0030] (1) Preparation of radish glycoside concentrate

[0031] Using radish seed meal as raw material, according to the ratio of mass (kg) of radish seed meal (kg): volume fraction of 50-80% ethanol solution (L) ratio of 1:6, the reaction was first stirred at room temperature for 4h, and then used A citric acid solution with a mass fraction of 2% was adjusted to pH 2, and then pumped into a clear tube centrifuge, centrifuged at a speed of 15000r / min, and centrifuged clear liquid and sediment were collected. For the collected centrifugal supernatant, pump into the reverse osmosis concentrator, and perform the first reverse osmosis concentration at 0.15 MPa until the volume of the first reverse osmosis retentate is reduced to 10% of the original volume. The first reverse osmosis permeate and the first reverse osmosis retentate. In the collected first reverse osmosis retentate, add deionized wate...

Embodiment 2

[0040] A method for preparing high-purity lysine, the specific method steps are as follows:

[0041] (1) Preparation of radish glycoside concentrate

[0042] Using radish seed meal as raw material, according to the ratio of mass (kg) of radish seed meal (kg): volume fraction of 65% ethanol solution (L) to ratio of 1:7, first stir and react at room temperature for 5h, and then use mass fraction Adjust the pH to 2.5 for a 4% citric acid solution, centrifuge at a speed of 18000 r / min, and collect the centrifuged supernatant and sediment. For the collected centrifugal supernatant, pump into the reverse osmosis concentrator, and perform the first reverse osmosis concentration at 0.20 MPa, until the volume of the first reverse osmosis retentate is reduced to 12% of the original volume, and collect the first The first reverse osmosis permeate and the first reverse osmosis retentate. In the collected first reverse osmosis retentate, add deionized water to make up to the original volume. ...

Embodiment 3

[0051] A method for preparing high-purity lysine, the specific method steps are as follows:

[0052] (1) Preparation of radish glycoside concentrate

[0053] Using radish seed meal as raw material, according to the ratio of mass (kg) of radish seed meal (kg): volume fraction of 80% ethanol solution (L) as 1:9, the reaction was first stirred at room temperature for 7 hours, and then mass fraction was used Adjust the pH to 3 for a 6% citric acid solution, centrifuge at a rotation speed of 20000 r / min, and collect the centrifuged supernatant and sediment. For the collected centrifugal supernatant, pump into the reverse osmosis concentrator, perform the first reverse osmosis concentration at 0.25 MPa, until the volume of the first reverse osmosis retentate is reduced to 15% of the original volume, collect the first The first reverse osmosis permeate and the first reverse osmosis retentate. In the collected first reverse osmosis retentate, add deionized water to make up to the origina...

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Abstract

The invention discloses a preparation method of high purity sulforaphene and belongs to the field of sulforaphene preparation. High purity sulforaphene is prepared from radish seed meal as a raw material by ethanol extraction, enzyme film reaction, chromatogram preparation and vacuum thin film evaporation. The preparation method has the characteristics of continuous production technology, simpleness, stability, high extraction and purification efficiency, high yield, high resource comprehensive utilization rate and no harmful substance discharge. In production, two enzymes are used as catalysts so that a sulforaphene enzymolysis yield is greatly improved. The preparation method of high purity sulforaphene has high purifying efficiency, realizes product purity of 98% or more and does not produce solvent residues. The high purity sulforaphene can be widely used in industries of medicines and health products.

Description

[0001] 1. Technical Field [0002] The invention belongs to the technical field of producing raisin, and particularly relates to a method for preparing high-purity raisin. [0003] 2. Technical background [0004] Glucosinolates (abbreviated as glucosinolates) are important secondary metabolites of cruciferous plants. Glucosinolates and their degradation products play an important role in plant disease resistance, special flavor composition and human health. Radish also contains glucosinolates, but the types of glucosinolates in radishes are different from other cruciferous plants. Radishes are mainly 4-methylsulfinyl-3-butenyl glucosinolates (commonly known as glucoraphenin). Radixin has the highest content in radish seeds and can be degraded by myrosinase to produce 4-methylsulfinyl-3-butenyl isothiocyanate (commonly known as sulforaphene). Studies have shown that lysine has extremely high biological activity in inhibiting cell mutation and anti-oxidation, and has great developmen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C331/22
Inventor 张杰余春阳王星敏刘帆邵承斌
Owner BEIKE HUAXIA BIOMEDICAL TECH CO LTD
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