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Preparation, purification and storage method of toxoplasma gondii oocysts

A preservation method, the technology of toxoplasma gondii, applied in the direction of protozoa, etc., can solve the problem of no effective method for the prevention and treatment of toxoplasmosis, and achieve the effect of simple and easy manual operation, small scope and easy control

Inactive Publication Date: 2016-02-03
HENAN AGRICULTURAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

[0005] At present, there is no effective method for the prevention and treatment of toxoplasmosis, so active research is also being carried out in this area

Method used

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  • Preparation, purification and storage method of toxoplasma gondii oocysts
  • Preparation, purification and storage method of toxoplasma gondii oocysts

Examples

Experimental program
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Embodiment 1

[0044] A. Acquisition of Toxoplasma gondii cysts:

[0045] Purchase 500 grams of fresh pork, 500 grams of chicken, 500 grams of fresh pig brain, and all the chicken heads, label the samples, make tissue compression tablets, make 10 compression tablets for each sample, and inspect the compression tablets in an "S" shape under a 10-fold microscope , Toxoplasma cysts were found in the tissue, the sample number was recorded, and marked as a positive sample for Toxoplasma gondii cysts. Toxoplasma gondii cysts are characterized by a round shape with a wall of 1 μm and dense banana-shaped bradyzoites inside the cyst.

[0046] B. Acquisition of Toxoplasma-negative cats:

[0047] Randomly purchase 2-month-old healthy cats from the pet market, collect 1 drop of foot blood in a small amount, suck the blood on filter paper, and use ELISA method (ELISA kit purchased from IDEXX) to detect anti-toxoplasma antibodies in the serum. Toxoplasma antibody-positive cats are sent to pets Rescue st...

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Abstract

The invention discloses a preparation, purification and storage method of toxoplasma gondii oocysts. The method comprises the following steps of A, acquiring a toxoplasma gondii cyst; B, acquiring a toxoplasma gondii negative cat; C, feeding the cat with the toxoplasma gondii cyst; D, collecting excrement from 1 to 21 days; E, separating the toxoplasma gondii oocysts from the excrement; F, storing the toxoplasma gondii oocysts. The toxoplasma gondii oocysts disclosed by the invention are good in activity, can be stored in a fridge of 4 degrees for a long time, and a storage period is longer than 5 years, a good research and preparation material for a toxoplasma gondii vaccine can be provided, a range is relatively small, and contacted materials and utensils are subjected to harmless treatment, so that environment pollution is avoided.

Description

technical field [0001] The invention belongs to the new technology in the field of toxoplasma gondii vaccine research, in particular to a method for preparing, purifying and preserving toxoplasma gondii oocysts. Background technique [0002] Toxoplasma gondii is an obligate intracellular parasite, which can parasitize in all nucleated cells except red blood cells and spread to all parts of the body with blood and lymph. Intermediate hosts are widespread and nonspecific, and definitive hosts are limited to cats or felines. Toxoplasmosis is a zoonotic disease with great harm, which belongs to the second category of animal diseases in China. [0003] Toxoplasma gondii is an important opportunistic pathogenic protozoan. Normal people and animals infected with Toxoplasma gondii usually show recessive infection and generally do not cause obvious symptoms, but they can remain latent in the body for a long time. In patients with defects (such as organ transplantation, AIDS and mal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/10
Inventor 杨玉荣王英华付晓莹郭锋冯永杰仲伟平郝立宾马超锋刘书平韦景平王凯陆瑶瑶
Owner HENAN AGRICULTURAL UNIVERSITY
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