Method for amplifying rice aroma gene Badh2 sequence

Abstract

The invention belongs to the technical field of molecular genetics, and particularly relates to a method for amplifying a rice aroma gene Badh2 sequence. The method for amplifying the rice aroma gene Badh2 sequence comprises the steps that 1, a specific primer set with the sequence shown in the SEQ ID No.1-26 is included; 2, basic group substitution or deficiency or addition is performed; 3, a polymerase chain reaction is performed in a PCR amplification system to obtain the rice aroma gene Badh2 sequence. The method has the advantages of being simple, rapid, low in technical requirement and accurate in result identification, can be used for identifying rice aromatic varieties and rice non-aromatic varieties, can also be used for identifying directive breeding rice aromatic varieties, can be used for genetic identification of conventional rice and bridge materials and plays an important role in various processes of molecular marker directive breeding rice varieties.

Description

Technical field [0001] The present invention belongs to the technical field of molecular genetics, and specifically relates to a method for amplifying the Badh2 sequence of rice fragrance gene. Background technique [0002] Fragrant rice is a kind of functional rice and special rice with a unique fragrance. Due to its good taste and significant medicinal effects, the fragrant rice enjoyed the name of "tribute rice" in ancient my country, and its international market price is much higher than that of non-fragrant rice. It is 2-3 times that of non-fragrant rice. With the increase in market demand, it is clear that the reason for the aroma of rice is an effective strategy to discover aroma gene resources and give full play to the advantages of aromatic rice, and it is the first choice for breeders to develop high-quality and characteristic rice varieties. As early as the early 1990s, the application of molecular markers located the aroma genes of rice on chromosome 8. However, it wa...

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Problems solved by technology

However, due to differences in climatic conditions and geographical locations, there are still many special local varieties of fragrant rice in my country, which are superior to other varieties in terms of nutrient components and trace element content, which cannot be detected by the four aroma genotype molecular markers that have been developed. In addition, while intensifying the research on aromatic rice varieties, it was found that the entire Badh2 gene sequence of these varieties could not be amplified using the reported primers, which hindered the mining of new aroma sources of aromatic rice and greatly affected the Breeding and Utilization of Fragrant Rice Resources

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