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Lentinan preparation method

A technology for lentinan and Lentinus edodes, which is applied in the field of functional ingredient extraction, can solve the problems of large molecular weight of lentinan, long extraction time, unfavorable development, etc., and achieves the effects of low extraction temperature, shortened extraction time, and preservation of physiological activity.

Inactive Publication Date: 2016-03-02
单联刚
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most manufacturers of lentinan in China use the traditional water extraction method, which takes a long time to extract and the molecular weight of the extracted lentinan is large, which is not conducive to the development of the market.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Dry the shiitake mushrooms in an oven at 40°C for 3 hours;

[0019] (2) crushed through a 60 mesh sieve;

[0020] (3) Take 20 g of crushed shiitake mushroom powder, add 400 mL of distilled water, then add 0.5 g of cellulase, and hydrolyze in a constant temperature water bath at 40°C for 60 min;

[0021] (4) heating up to 50° C., adjusting the pH of the solution to 5.5, extracting for 20 minutes under ultrasonic conditions with a power of 200 W, and then filtering to obtain an extract;

[0022] (5) Cool the extraction solution to 40°C with running water, add 0.5% trypsin, stir well and let stand for 20 minutes;

[0023] (6) flowing water cooling extract solution to room temperature;

[0024] (7) Centrifuge at a speed of 3000r / min for 15min, and take the supernatant;

[0025] (8) After adding 2 times the volume of absolute ethanol, refrigerate at 4°C for 5 hours;

[0026] (7) Centrifuge at a speed of 3000 r / min for 15 min to obtain a precipitate, put it into an ov...

Embodiment 2

[0028] (1) Dry the shiitake mushrooms in an oven at 60°C for 7 hours;

[0029] (2) crushed through 80 mesh sieves;

[0030] (3) Take 20 g of crushed shiitake mushroom powder, add 600 mL of distilled water, then add 0.5 g of cellulase, and hydrolyze in a constant temperature water bath at 50°C for 90 min;

[0031] (4) heating up to 70° C., adjusting the pH of the solution to 5.5, extracting for 40 minutes under ultrasonic conditions with a power of 400 W, and then filtering to obtain an extract;

[0032] (5) Cool the extraction solution to 50°C with running water, add 0.5% trypsin, stir well and let stand for 40 minutes;

[0033] (6) flowing water cooling extract solution to room temperature;

[0034] (7) Centrifuge at a speed of 3000r / min for 15min, and take the supernatant;

[0035] (8) After adding 4 times the volume of absolute ethanol, refrigerate at 4°C for 15 hours;

[0036] (7) Centrifuge at a speed of 3000 r / min for 15 min to obtain a precipitate, put it into an ov...

Embodiment 3

[0038] (1) Dry the shiitake mushrooms in an oven at 50°C for 6 hours;

[0039] (2) crushed through a 70 mesh sieve;

[0040] (3) Take 20 g of crushed shiitake mushroom powder, add 500 mL of distilled water, and then add 0.5 g of cellulase, and hydrolyze in a constant temperature water bath at 45°C for 80 min;

[0041] (4) Heating up to 60°C, adjusting the pH of the solution to 5.5, extracting for 30 minutes under ultrasonic conditions with a power of 2300W, and then filtering to obtain an extract;

[0042] (5) Cool the extraction liquid under running water to 45°C, add 0.5% trypsin, stir well and let stand for 30 minutes;

[0043] (6) flowing water cooling extract solution to room temperature;

[0044] (7) Centrifuge at a speed of 3000r / min for 15min, and take the supernatant;

[0045] (8) After adding 3 times the volume of absolute ethanol, refrigerate at 4°C for 10 hours;

[0046] (7) Centrifuge at a speed of 3000 r / min for 15 min to obtain a precipitate, put it into an ...

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Abstract

The invention relates to a lentinan preparation method which comprises the following steps: utilizing cellulase for preliminary hydrolysis of a lentinus edodes powder extract, and then performing polysaccharide extraction under the ultrasonic condition. According to the method, ultrasonic cavitation and mechanical action are utilized for breaking cell walls of lentinus edodes, and then the movement of polysaccharide diffusion and dissolution is accelerated under the action of ultrasonic high-frequency vibration, so as to greatly promote outward diffusion of intracellular polysaccharides, and shorten the polysaccharide extraction time; meanwhile, the extraction temperature for the method is low, so that the physiological activity of lentinan can be preserved to a relatively great extent.

Description

(1) Technical field [0001] The invention relates to a method for preparing lentinan, which belongs to the technical field of functional component extraction. (2) Background technology [0002] Mushrooms (Lentinusedodes) belong to Basidiomycetes, Agaricales, Pleurotaceae, and Lentinus genus plants, which are widely planted around the world, accounting for 25% of the world's total output of mushroom trees, and are also the second most popular edible fungi in the world. Because of its delicious taste, rich nutrition and medicinal value, it is loved by people. [0003] Lentinan is an important effective medicinal ingredient in Lentinus edodes, which has biologically active functions such as anti-virus, anti-tumor, anti-radiation, and anti-aging. At present, most manufacturers of lentinan in China use the traditional water extraction method, which takes a long time to extract and the molecular weight of the extracted lentinan is large, which is not conducive to the development o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0024C08B37/0003
Inventor 单联刚
Owner 单联刚