Induction method of inflorescence callus of Z.
A technology of callus and eelgrass flower branch, which is applied in the field of induction of inflorescence callus of eelweed in vitro, can solve the problems of patents and literatures that have not been reported, and achieve the effect of improving the surface disinfection effect and shortening the acquisition time
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Embodiment 1
[0015] Embodiment 1: a kind of induction method of eelgrass callus, its implementation step comprises:
[0016] 1) Selection of explants: collect the flowering branches of Zinaria cerevisiae, take the healthy and young spathe inflorescences, scrape off the attached organisms on the surface, wash them in seawater, and transfer them to the ultra-clean workbench. Soak in hydrogen peroxide solution for 15-20 minutes, rinse with sterilized sea water for 3 times, peel off the bract leaves outside spathe inflorescences to obtain sterile inflorescences;
[0017] 2) Induction culture of callus: cut the sterile inflorescence into 3-5mm long explant fragments, inoculate them in the induction medium, culture them at 20°C in the dark, and transfer them every half month. After 28 days, the tissues on both sides of the inflorescence germinated and began to expand, and gradually formed callus, and the induction rate was as high as 34%;
[0018] 3) The improved MS medium is based on MS medium...
Embodiment 2
[0019] Embodiment 2: a kind of induction method of eelgrass callus, its implementation steps are:
[0020] 1) Selection of explants: Take healthy and young spathe inflorescences from the flower branches of Echinopsis euphratica, scrape off the attached organisms on the surface, wash them in sea water, transfer them to a clean bench, and use 10% hydrogen peroxide to clean them. Soak in the solution for 15-20 minutes, rinse with sterilized seawater for 3 times, and peel off the bract leaves outside the spathe inflorescence to obtain a sterile inflorescence;
[0021] 2) Induction culture of callus: cut the sterile inflorescence into 3-5mm long explant fragments, inoculate them in the induction medium, culture them at 20°C in the dark, and transfer the bottles once every half month. After 40 days, the tissues on both sides of the inflorescence sprouted and began to expand, gradually forming callus, and the induction rate was 26%;
[0022] 3) The improved MS medium is based on MS ...
Embodiment 3
[0023] Embodiment 3: a kind of induction method of eosinopsis somatic embryo, its implementation steps are:
[0024] 1) Selection of explants: Take healthy and tender spathe inflorescences from the flower branches of Echinopsis euphratica, scrape off the attached organisms on the surface, wash them in seawater, transfer them to a clean workbench, and use 10% hydrogen peroxide to clean them. Soak in the solution for 15-20 minutes, rinse with sterilized seawater for 3 times, and peel off the bract leaves outside the spathe inflorescence to obtain a sterile inflorescence;
[0025] 2) Induction culture of callus: cut the sterile inflorescence into 3-5mm long explant fragments, inoculate them in the induction medium, culture them at 20°C in the dark, and transfer them every half month. After 40 days, the tissues on both sides of the inflorescence germinated, began to expand, and callus gradually formed, with an induction rate of 7%;
[0026] 3) The improved MS medium is based on M...
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