TRPC3 (Transient receptor potential cation channel, subfamily C, member 3) antigen peptide and anti-TRPC3 monoclonal antibody

A monoclonal antibody and antigen polypeptide technology, applied in the field of cell engineering, can solve the problem of no immunogenic antigen polypeptide, etc., and achieve the effect of easy human processing and quality control, single biological activity and strong specificity

Inactive Publication Date: 2016-03-16
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, to prepare antibodies that can specifically bind TRPC3 protein, it is first necessary to screen the appropriate TRPC3 polypeptide sequence to obtain a good immunogen, but currently there is no antigenic polypeptide with good immunogenicity and high specificity

Method used

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  • TRPC3 (Transient receptor potential cation channel, subfamily C, member 3) antigen peptide and anti-TRPC3 monoclonal antibody
  • TRPC3 (Transient receptor potential cation channel, subfamily C, member 3) antigen peptide and anti-TRPC3 monoclonal antibody

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Experimental program
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Effect test

Embodiment 1

[0026] Embodiment 1, TRPC3 Antigen Peptide Design

[0027] TRPC3 antigen polypeptide YFTYARDKWLPSDPQ (SEQ ID NO: 1)

[0028] The above-mentioned TRPC3 antigen polypeptide α -spiral, β -Turn angle, hydrophilicity and antigenic index are detected, and its detection result is: the antigenic index prediction of the TRPC3 antigenic polypeptide provided by the present invention is 0.995, and revealing predictive value is 1.000, and flexibility predictive value is 1.003, and β-rotation angle predictive value is 1.080, B Cell epitope prediction is better. That is, the TRPC3 antigen polypeptide α -spiral, β -Turning angle, hydrophilicity and antigenic index are all in line with the results of B cell epitope analysis, among which the antigenic index of TRPC3 antigenic polypeptide has a significant advantage, see the results figure 1 shown.

Embodiment 2

[0029] Example 2 1. Preparation of monoclonal antibody against TRPC3

[0030] 1. Test objects: 4 healthy BALB / c mice were selected, and the age of the mice was 6-8 weeks.

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Abstract

The invention discloses a TRPC3 (Transient receptor potential cation channel, subfamily C, member 3) antigen peptide and an anti-TRPC3 monoclonal antibody. The TRPC3 antigen peptide comprises 15 amino acids, and a specific sequence is represented as SEQ ID NO: 1. A preparation method of the anti-TRPC3 monoclonal antibody comprises steps as follows: the TRPC3 antigen peptide after being coupled with KLH (keyhole limpet hemocyanin) is taken as an antigen for immunization of a mouse, a hybridoma cell strain T-II is obtained through screening, and the anti-TRPC3 monoclonal antibody is obtained through secretion of the hybridoma cell strain T-II. The anti-TRPC3 monoclonal antibody can specifically recognize TRPC3 channel protein expressed by various tissue cells and can be applied to TRPC3 expression research of various tissue cells, thereby monitoring diseases related with TRPC3 molecules. Therefore, the anti-TRPC3 monoclonal antibody can be applied to preparation of diagnostic reagents for diseases related with TRPC3 molecule expression.

Description

technical field [0001] The invention relates to the technical field of cell engineering, in particular to a TRPC3 antigen polypeptide and an anti-TRPC3 monoclonal antibody. Background technique [0002] Atherosclerosis (AS) is the most common type of arteriosclerotic vascular disease, which is characterized by the involvement of arterial lesions starting from the intima. The general formation process of AS is the accumulation of lipids and complex carbohydrates, hemorrhage and thrombus formation, fibrous tissue hyperplasia and calcium deposition, followed by gradual degeneration and calcification of the arterial media, and the lesions often involve elastic and medium-sized muscular arteries. Once it develops enough to block the lumen of the artery, the tissue or organ supplied by the artery will be ischemic or necrotic. Symptoms of atherosclerosis are mainly determined by vascular lesions and the degree of ischemia of the affected organs. In patients with coronary atheroscl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08C12N5/20C07K16/18G01N33/68G01N33/577C12R1/91
Inventor 张萃刘晓波郭雨青邹琴
Owner GUANGDONG PHARMA UNIV
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