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Composite microbial agent, and preparation method and application thereof

A technology for compounding microbial inoculants and seeds is applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve the effects of improving specificity, simple use and low cost

Inactive Publication Date: 2016-03-16
北京沃土天地生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the high content of organic matter, COD and harmful substances in Chinese herbal medicine waste liquid, there is a high requirement for the tolerance of microorganisms. So far, there is no special microbial agent for the treatment of Chinese herbal medicine waste liquid.

Method used

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  • Composite microbial agent, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 is used to illustrate the composite microbial bacterial agent of the present invention.

[0038] The compound microbial bacterial agent comprises:

[0039] Bacillus subtilis VTS-2 (Bacillus subtilis), the number of viable bacteria is 1×10 8 -6×10 8 cfu / mL;

[0040] Bacillus megaterium is VTS-1 (Bacillus megatherium), the number of live bacteria is 1.5×10 7 -5×10 7 cfu / mL;

[0041] Pichia pastoris VTJ-6 (Pichia pastoris), the number of viable bacteria is 2×10 8 -4×10 8 cfu / mL;

[0042] Absidia VTJ-3 (Absidia), the number of viable bacteria is 1×10 6 -4×10 6 cfu / mL.

Embodiment 2

[0044] Example 2 is used to illustrate the preparation method of the composite microbial bacterial agent of the present invention.

[0045] Including the following steps:

[0046] 1) Solid slope culture Inoculate Bacillus subtilis VTS-2 (Bacillus subtilis) and Bacillus megaterium VTS-1 (Bacillus megatherium) in beef extract peptone solid medium, Pichia pastoris VTJ-6 (Pichia pastoris), Mucor (Ploughshare Mycobacterium VTJ‐3 (Absidia)) was inoculated in PDA solid medium, and cultured at 28°C for 1 day to activate the strain;

[0047] Among them, the formula of beef extract-peptone solid medium is 5g of beef extract, 10g of peptone, 5g of sodium chloride, 1000mL of water, and 20g of agar; the formula of PDA solid medium is 200g of potatoes, 20g of glucose, 1000mL of water, and 20g of agar.

[0048] 2) Primary seed cultivation: Inoculate the activated Bacillus subtilis and Bacillus megaterium strains in the beef extract peptone medium in step 1), inoculate the activated Pichia p...

Embodiment 3

[0057] Example 3 is used to illustrate another optional preparation method of the composite microbial agent of the present invention.

[0058] Including the following steps:

[0059] 1) Solid slant culture Inoculate Bacillus subtilis and Bacillus megaterium in beef extract peptone solid medium, inoculate Pichia pastoris and mucormyces in PDA solid medium, and culture at 29°C for 2 days to activate the strains;

[0060] Among them, the formula of beef extract-peptone solid medium is 5g of beef extract, 10g of peptone, 5g of sodium chloride, 1000mL of water, and 20g of agar; the formula of PDA solid medium is 200g of potatoes, 20g of glucose, 1000mL of water, and 20g of agar.

[0061] 2) Primary seed cultivation: Inoculate the activated Bacillus subtilis and Bacillus megaterium strains in the beef extract peptone medium in step 1), inoculate the activated Pichia pastoris and Mucor in the PDA medium, at 29°C, Cultivate at 170rpm for 3 days, when the density of liquid culture bac...

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Abstract

The invention relates to a composite microbial agent, specifically to a composite microbial agent used for treating Chinese herbal medicine waste liquid, and a preparation method and application thereof. The composite microbial agent comprises Bacillus subtilis, Bacillus megaterium, Pichia yeast and hair mould and has a total live bacterium number of no less than 3 to 10<8> cfu / mL. Directed at components of the Chinese herbal medicine waste liquid, the composite microbial agent adopts reasonable bacterial strain composition, most harmful substances are degraded by corresponding bacterial strains, so the specificity of the microbial agent is effectively improved; and Substances like COD and BOD in the Chinese herbal medicine waste liquid are effectively decomposed by a plurality of enzymes produced by the composite microbial agent.

Description

technical field [0001] The invention relates to a composite microbial bacterial agent, in particular to a composite microbial bacterial agent for treating Chinese herbal medicine waste liquid. Background technique [0002] Chinese herbal medicine is a traditional treasure of the Chinese nation. Because of its unique efficacy, it has an important position in world medicine. With the development of the times, Chinese herbal medicine pharmaceuticals in my country have developed rapidly. According to statistics, there are about 2,000 Chinese herbal medicine manufacturers on the market. Chinese herbal medicine produces a large amount of waste liquid during the pharmaceutical production process, most of which are directly discharged or enter the sewage treatment system. Chinese herbal medicine waste liquid contains a large amount of organic matter, as well as sugars, lignin, alkaloids and other pollutants. Direct discharge will pollute groundwater, and the odor will seriously aff...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C12N1/14C05F11/08C12R1/125C12R1/10C12R1/84C12R1/65
CPCC12N1/20C05F11/08C12N1/14C12N1/16
Inventor 杨军任莉彭生平
Owner 北京沃土天地生物科技股份有限公司
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