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Method for increasing detecting and recovery rate of melamine in milk product

A technology for melamine and third melamine is applied in the field of detecting melamine in dairy products and improving the detection recovery rate of melamine in dairy products, which can solve problems such as low recovery rate compliance rate, shorten time, improve work efficiency, and improve detection recovery rate. Effect

Inactive Publication Date: 2016-03-16
INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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Problems solved by technology

[0003] Use the national standard method to detect melamine in dairy products. When the recommended concentration point is 2mg / kg, 4mg / kg, 10mg / kg or 20mg / kg each time, the coincidence rate of the determined recovery rate is low and the recovery rate is average. It takes multiple tests to reach the range of 80%-110% required by the national standard GB / T22388-2008 "Measurement Method for Melamine in Raw Milk and Dairy Products"

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  • Method for increasing detecting and recovery rate of melamine in milk product
  • Method for increasing detecting and recovery rate of melamine in milk product

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Experimental program
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Embodiment

[0024] 1. Methods for detecting melamine in dairy products generally include:

[0025] 1. Test consumables

[0026] 1.1 Reagents and materials: Methanol (chromatographically pure), acetonitrile (chromatographically pure), ammonia water (content 25%-28%) trichloroacetic acid, citric acid, sodium octane sulfonate (chromatographically pure), methanol water (1:1) , trichloroacetic acid (1%), ammoniated methanol (5%), mobile phase (ion-pair reagent buffer: acetonitrile=90:10): melamine standard (2ug / ml), melamine standard stock solution (1000ug / ml ), cation exchange solid phase extraction column (60mg / 3ml), qualitative filter paper, sea sand, microporous membrane, nitrogen.

[0027]1.2 Instruments and equipment: high-performance liquid chromatography (HPLC) instrument, molecular balance, high-speed centrifuge, ultrasonic cleaning machine, solid-phase extraction device, nitrogen drying system, oscillator, plastic centrifuge tube with plug, mortar and bowl.

[0028] 2. Sample pretr...

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Abstract

The invention discloses a method for detecting melamine in a milk product. The method comprises the steps that melamine in a sample is extracted, and an extracting solution is obtained, wherein melamine in the sample is ultrasonically extracted through trichloroacetic acid-acetonitrile, the first extracting solution is obtained, first centrifugation is carried out on the first extracting solution, first supernate is obtained and filtered, filter liquor is obtained, second centrifugation is arranged on the filter liquor, second supernate is obtained, and the centrifugal speed of first centrifugation and the centrifugal speed of second centrifugation are not lower than 4,000 r / min; the extracting solution is purified, purified liquor is obtained and a solid-phase extraction column is adopted during purification; high performance liquid chromatography is utilized for detecting the purified liquor to detect melamine in the milk product. The invention further discloses a method for increasing the detecting and recovery rate of melamine. By means of the method, the detecting and recovery rate meeting the national standard can be conveniently obtained.

Description

technical field [0001] The present invention relates to the field of food detection. Specifically, the present invention relates to a method for improving the detection and recovery rate of melamine in dairy products. More specifically, the present invention provides a method for detecting melamine in dairy products and a method for improving the detection rate of melamine in dairy products. Method for detecting recovery. Background technique [0002] The detection of melamine in dairy products adopts the national standard GB / T22388-2008 "Detection method for melamine in raw milk and dairy products". [0003] Use the national standard method to detect melamine in dairy products. When the recommended concentration point is 2mg / kg, 4mg / kg, 10mg / kg or 20mg / kg each time, the coincidence rate of the determined recovery rate is low and the recovery rate is average. It takes multiple tests to reach the range of 80%-110% required by the national standard GB / T22388-2008 "Measurement...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 焦颖佳韩丽娜
Owner INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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