Antibody, method and kit for detecting and determining phillyrin

Abstract

The invention provides a hapten comprising phillyrin or a derivative thereof, an immunogen of the hapten which is combined with a carrier substance endowing the antigenicity, a conjugate (a coating antigen) of the hapten combined with a labeling reagent and an antibody of the immunogen. The antibody can be combined with at least one structural antigenic determining part in intact phillyrin. The invention further provides a method and a kit for detecting or quantifying phillyrin in a sample and use of the conjugate and the antibody in detecting or quantifying phillyrin. The antibody, the method and the kit provided by the invention are specific for phillyrin and can be used for detecting existence of phillyrin in the sample and determining the content of phillyrin.

Description

technical field [0001] The invention relates to an antibody of a small molecular hapten and its preparation method and application, in particular to an antibody of forsythin and its derivatives and its preparation method and application. Background technique [0002] The present invention relates to methods and kits for detection and quantification of forsythin, and haptens, immunogens, conjugates and antibodies used therein. [0003] Where "detection" is the presence or absence of a specified analytical substance. [0004] Where "determination" refers to the quantitative analysis of a substance. [0005] Forsythin is an extract derived from the dried fruit of Forsythia oleaceae. [0006] Molecular formula: C 27 h 34 o 11 Molecular weight: 534.56 [0007] Forsythin belongs to the glucoside of diepoxide lignans, with a melting point of 184°C to 185°C. [0008] Studies have shown that forsythin shows a variety of biological activities and pharmacological effects. It ha...

AI Technical Summary

Problems solved by technology

However, biological samples containing forsythin (including plasma, urine, saliva, etc.) contain a large amount of proteins and endogenous substances that affect the determination of the content; at the same time, forsythin may be in a combined state and must be processed to eliminate endogenous impurities and The interference of metabolites makes the binding state of forsythin free before it can be measured; at the same time, it needs to be concentrated to meet the requirements of instrument detection sensitivity, the processing procedure is complicated, time-consuming and laborious; in addition, after entering the body, the distribution of forsythin in the tissue is Extremely small amount, even after enrichment, it is still extremely difficult to determine the content

Finally, for the distribution of forsythin in target organs and tissues, as well as the study of cellular and subcellular localization, methods such as immunohistochemistry and westblot are required, but the lack of antibodies to forsythin hinders this research

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