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Method for preparing polyphenol polymers with alpha-glucosidase inhibiting activity from teng tea

A technology of polyphenol polymer and glucosidase, which is applied in the direction of pharmaceutical formulations, plant raw materials, medical preparations containing active ingredients, etc., can solve problems such as diarrhea and abdominal discomfort, and achieve high safety, simple production process, and broad The effect of market prospects

Active Publication Date: 2016-04-06
HENAN INST OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these drugs have obvious side effects such as flatulence, diarrhea, and abdominal discomfort.

Method used

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  • Method for preparing polyphenol polymers with alpha-glucosidase inhibiting activity from teng tea
  • Method for preparing polyphenol polymers with alpha-glucosidase inhibiting activity from teng tea
  • Method for preparing polyphenol polymers with alpha-glucosidase inhibiting activity from teng tea

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Weigh 40g of vine tea, add 2000mL boiling water, soak for 0.5h, freeze-dry to obtain 15.12g of vine tea extract, dissolve vine tea extract in 100mL of 50% methanol aqueous solution (v / v), and fill with SephadexLH 20 chromatographic column (column volume is about 100mL), first use 1000mL of 50% methanol aqueous solution (v / v) to wash away impurities, and then use 300mL of 70% acetone aqueous solution (v / v) to elute, and wash the obtained The liquid was removed and placed in a dialysis bag with a molecular weight cut-off of 1000, dialyzed with pure water for 24 hours, and the water was changed 3 times in the middle, and finally the sample liquid in the bag was collected, freeze-dried, and the polyphenolic polymer was 503 mg.

Embodiment 2

[0017] Weigh 40g of vine tea, add 1600ml of boiling water, soak for 1 hour, and freeze-dry to obtain 14.75g of vine tea extract. Dissolve the vine tea extract in 100mL of 50% methanol aqueous solution (v / v), fill it with SephadexLH-20 chromatography column (column volume is about 100mL), first wash with 500mL of 70% methanol aqueous solution (v / v) Remove impurities, then elute with 400mL 80% acetone aqueous solution (v / v), put the obtained aqueous solution into a dialysis bag with a molecular weight cut-off of 1000, dialyze with pure water for 48h, and change the water 6 times in between. Finally, the sample liquid in the bag was collected, freeze-dried, and the polyphenolic polymer was 485mg.

Embodiment 3

[0019] Weigh 40g of vine tea, add 1200mL of boiling water, soak for 0.5h, and freeze-dry to obtain 12.14g of vine tea extract. Dissolve the vine tea extract in 100mL 50% methanol aqueous solution (v / v), put it on a chromatographic column filled with SephadexLH-20 (column volume is about 100mL), and wash it away with 500mL 60% methanol aqueous solution (v / v) Impurities were then eluted with 300 mL of 90% acetone aqueous solution (v / v), and the resulting aqueous solution was placed in a dialysis bag with a molecular weight cut-off of 1000, dialyzed with pure water for 48 hours, and the water was changed 3 times in the middle, and finally the sample liquid in the bag was collected and frozen Drying, polyphenolic polymer 465mg.

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Abstract

The invention provides a method for preparing polyphenol polymers with alpha-glucosidase inhibiting activity from teng tea. The method for preparing polyphenol polymers with alpha-glucosidase inhibiting activity from teng tea is characterized by comprising the following steps of (1) teng tea water extract preparation: putting the teng tea into boiling water, soaking the teng tea for a period of time, filtering liquid by gauze, and performing freeze drying on filter liquid to obtain a teng tea water extract; (2) column chromatography separation: dissolving the teng tea extract by a 50-percent methanol water solution, using an upper chromatography column, firstly flushing away impurities by the methanol water solution with a certain concentration, then performing elution by an acetone water solution with a certain concentration, and collecting an elution solution; and (3) membrane dialysis: performing vacuum rotary evaporation on the obtained elution solution to remove acetone, putting the obtained water solution into a dialysis bag, performing dialysis by water for a certain time, collecting a sample solution in the bag, performing freeze drying and obtaining the polyphenol polymers. The method has the advantages that the production process is simple, and the cost is low.

Description

Technical field: [0001] The invention relates to a method for extracting polyphenol polymers, in particular to a method for preparing polyphenol polymers with alpha-glucosidase inhibitory activity from vine tea. Background technique: [0002] Diabetes is a disease characterized by a metabolic disorder that fails to efficiently transport glucose from the blood to cells. It mainly includes congenital type 1 diabetes and acquired type 2. Type 1 diabetes can be diagnosed in childhood and does not secrete insulin in the body. Type 2 diabetes is caused by insulin resistance and is mainly found in adults. Diabetic patients90 % above are of this type. Mammalian α-glucosidase is located on the surface of small intestinal villi cells and is a key enzyme for carbohydrate digestion and absorption. Its main function is to hydrolyze oligosaccharides or disaccharides into monosaccharides for human absorption. Therefore, α-glucosidase inhibitors can delay the hydrolysis of oligosaccharide...

Claims

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Application Information

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IPC IPC(8): A61K36/87
Inventor 刘本国陈玉珍耿升刘长忠马汉军
Owner HENAN INST OF SCI & TECH
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