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Determination method of vitamin a, e and corresponding online solid phase extraction analysis system

A solid-phase extraction column and vitamin technology, applied in the field of online solid-phase extraction analysis system, can solve the problems of time-consuming, easily lost components to be measured, and large error in sample determination results

Active Publication Date: 2018-03-27
COFCO NUTRITION & HEALTH RES INST +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although this method has a good purification effect and less interference from impurities, it has disadvantages such as time-consuming operation, large consumption of organic solvents, and easy loss of components to be measured.
Due to the multiple extraction, concentration and dissolution steps involved, the operation is cumbersome and time-consuming, and it is easy to cause the loss of the components to be tested. The accuracy of the data is closely related to the experimental skills of the operator, and the error of the sample measurement results is large and the reproducibility is not good.

Method used

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  • Determination method of vitamin a, e and corresponding online solid phase extraction analysis system
  • Determination method of vitamin a, e and corresponding online solid phase extraction analysis system
  • Determination method of vitamin a, e and corresponding online solid phase extraction analysis system

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Embodiment 1

[0027] (1) Instruments and reagents

[0028] Ultimate3000 dual ternary liquid chromatography system (Thermo Scientific Company), equipped with dual ternary gradient pumps, autosampler, column thermostat with a six-way valve, diode array detector, Chameleon chromatography management software; Milli-Q ultrapure Water machine (Millipore Company); desktop high-speed refrigerated centrifuge (Thermo Scientific Company).

[0029] Vitamin A (retinol), α-tocopherol, β-tocopherol, γ-tocopherol and δ-tocopherol standard (Sigma company); potassium hydroxide, ascorbic acid (analytical grade, Sinopharm Beijing Chemical Reagent Co., Ltd. ); absolute ethanol and methanol (chromatographic grade, Fisher Company); other reagents were of analytical grade; absolute ethanol was used after dealdehyde treatment; pig liver, eggs and infant formula milk powder were purchased from local supermarkets.

[0030] (2) Chromatographic conditions

[0031]The analytical column is Zorbax Extend-C18 (4.6mm×150m...

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Abstract

The object of the present invention is to provide a method for determining vitamin A and vitamin E in animal source food. Specifically, the present invention uses solid-phase extraction and high-performance liquid chromatography to establish an online solid-phase extraction-high-performance liquid chromatography analysis method for simultaneously determining vitamin A and vitamin E in animal-derived foods. In addition, the invention also provides an online solid-phase extraction analysis system for detecting vitamin A and vitamin E.

Description

technical field [0001] The invention relates to a method for measuring vitamin A and vitamin E in animal source food. Specifically, the present invention adopts solid-phase extraction and high-performance liquid chromatography to establish an online solid-phase extraction-high performance liquid chromatography analysis method for simultaneous determination of vitamin A and vitamin E in animal-source foods. In addition, the invention also provides an online solid-phase extraction analysis system for detecting vitamin A and vitamin E. Background technique [0002] Vitamin A and vitamin E are micronutrients necessary for the body to maintain normal physiological functions and material and energy metabolism. They have biological effects such as regulating growth, maintaining vision, promoting reproduction, and delaying aging. However, they cannot be synthesized in the body or the amount of synthesis is not enough to satisfy the body. If needed, it must be supplemented with food...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/20
Inventor 谢云峰王浩杨永坛刘佟任丹丹
Owner COFCO NUTRITION & HEALTH RES INST
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