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Sugar solution production method

A manufacturing method and technology of sugar solution, applied in biofuels, fermentation, etc., can solve the problems of low enzyme reusability, increased manufacturing cost of sugar solution, high price of cellulase, etc., to achieve efficient recovery, control of manufacturing costs, and reduction of The effect of usage

Active Publication Date: 2019-05-14
TORAY IND INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the disadvantage of the method of producing sugar solution using cellulase is that the production cost of sugar solution increases due to the high price of cellulase
In order to solve such technical problems, a method of recovering and reusing cellulase used in hydrolysis has been proposed. However, since cellulase is strongly adsorbed to the hydrolysis residue generated when hydrolyzing cellulose-containing biomass, the reuse of the enzyme is difficult. low sex becomes a problem

Method used

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Effect test

reference example 1

[0066] (Reference Example 1) Measurement of Protein Concentration

[0067] For the measurement of the protein concentration, a commercially available protein concentration measurement reagent (Quick Start Bradford Protein Assay, manufactured by Bio-Rad) was used. Add 5 μL of diluted cellulase solution to 250 μL of the protein concentration measurement reagent returned to room temperature, and after standing at room temperature for 5 minutes, measure its concentration at 595 nm with a microplate reader (POWERSCAN HT, manufactured by Dainippon Sumitomo Pharmaceutical Co., Ltd.). Absorbance. The bovine serum albumin aqueous solution was used as a standard solution, and the protein concentration of the cellulase solution was calculated with reference to the standard curve.

reference example 2

[0068] (Reference example 2) Measurement of sugar concentration

[0069] The concentrations of glucose and xylose contained in the sugar solution were quantified by comparison with standard products under the HPLC conditions shown below.

[0070] Column: Luna NH 2 (manufactured by Phenomenex)

[0071] Mobile phase: Milli-Q:acetonitrile=25:75 (flow rate 0.6mL / min)

[0072] Detection method: RI (differential refractive index)

[0073] Temperature: 30°C.

reference example 3

[0074] (Reference Example 3) Method for Measuring Cellulase Activity Derived from Filamentous Fungi

[0075] Cellulase activity, that is, the activity of an enzyme group involved in the decomposition of cellulose, was measured and evaluated by the method shown below (1) 4-nitrophenyl-β-D-lactopyranoside (pNP-Lac) and (2) the decomposing activity of 4-nitrophenyl-β-D-glucopyranoside (pNP-Glc); as the activity of enzyme group related to the decomposition of xylan which is the main component of hemicellulose, by (3) The decomposition activity of 4-nitrophenyl-β-D-xylopyranoside (pNP-Xyl) was measured and evaluated by the method shown below. In addition, the substrates of (1) to (3) above are collectively referred to as pNP-sugar.

[0076] Add 0.1 mL of enzyme solution to 0.9 mL of 55 mM acetate buffer (pH 5.0) containing each substrate at a concentration of 1.1 mM, and react at 30°C (final concentration of substrate 1 mM, final concentration of buffer 50mM). In the case of sub...

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Abstract

The present invention relates to a method for producing sugar liquid, comprising the following steps (1) to (3), step (1): pretreating the first cellulose-containing biomass, step (2): adding the second cellulose-containing biomass The substance is hydrolyzed by cellulase derived from filamentous fungi, and the amount of the second cellulose-containing biomass relative to the solid content weight of the first cellulose-containing biomass pretreated product of step (1) is 1 ~50% range, step (3): separate the hydrolyzate from step (2) into solution components and hydrolysis residues, filter the solution components through an ultrafiltration membrane, and recover filamentous bacteria as impermeable liquid The cellulase is used as the permeate to recover the sugar solution.

Description

technical field [0001] The present invention relates to a method for producing sugar liquor from cellulose. Background technique [0002] In recent years, methods for hydrolyzing biomass using cellulase have been extensively studied because of their low energy usage and environmental load and their high sugar yield. However, the production method of the sugar solution using cellulase has a disadvantage in that the production cost of the sugar solution increases due to the high price of cellulase. In order to solve such technical problems, a method of recovering and reusing the cellulase used in hydrolysis has been proposed, but since the cellulase is strongly adsorbed to the hydrolysis residue generated when hydrolyzing cellulose-containing biomass, the reuse of the enzyme is difficult. Low sex becomes a problem. [0003] As a method of desorbing the cellulase adsorbed on the hydrolysis residue and improving the recovery rate of the cellulase, a method of washing the hydro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/02C12P7/06C12P7/56
CPCC12P7/56C12P19/14C12P2201/00C12P19/02C12P2203/00Y02E50/10
Inventor 山田千晶栗原宏征山田胜成
Owner TORAY IND INC