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Bioremediation method of oil-contaminated soil

A technology for oil pollution and bioremediation, applied in the field of bioremediation of oil-contaminated soil, can solve the problems of limited and single treatment effect, and achieve the effects of improving bioavailability, increasing nutrient uptake, and reducing treatment costs

Inactive Publication Date: 2016-04-13
JINAN JIAXING TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, so far, most of the existing technologies use a single microbial remediation or phytoremediation technology to treat oil-contaminated soil, and its treatment effect is limited.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] In the bioremediation method of petroleum-contaminated soil in this example, 1 strain of C. chinensis and 1 strain of Pseudomonas were used to prepare a liquid mixed microbial preparation. The preparation method is as follows:

[0032] Inoculate C. chinensis in a 300mL Erlenmeyer flask with 100mL liquid potato culture medium, cultivate it for 3 days at 28°C and 160rpm, collect the culture, and the fungus content is 20mg dry weight / mL; The volume of 100mL LB liquid medium is 300mL Erlenmeyer flask inoculated with Pseudomonas, cultivated at 30°C and 160rpm for 1 day, collected the culture, and the bacterial content was 10 8 Individual / mL; the obtained fungal culture and bacterial culture were mixed according to a mixing ratio of 10:1 to obtain the liquid mixed microbial preparation used in the examples.

[0033] Then add 0.51kg / m to oil-contaminated soil 2 The liquid mixed microbial preparation, the SDS surfactant that accounts for 3% of the soil mass percentage, the pol...

Embodiment 2

[0037] In the bioremediation method of petroleum-contaminated soil in this example, 1 strain of Phanerochaete chrysosporium and 1 strain of Rhodococcus were used to prepare a liquid mixed microbial preparation, and the preparation method was as follows:

[0038] Inoculate Phanerochaete chrysosporium in a 300mL Erlenmeyer flask with a volume of 100mL liquid potato medium, cultivate it for 3 days at 30°C and 170rpm, collect the culture, and the fungal content is 60mg dry weight / mL; The volume of 100mL LB liquid medium is 300mL Erlenmeyer flask inoculated with Pseudomonas, cultivated at 30°C and 200rpm for 2 days, collected the culture, and the bacterial content was 10 12 Individual / mL; The obtained fungal culture and bacterial culture were mixed according to a mixing ratio of 1:1 to obtain the liquid mixed microbial preparation used in the examples.

[0039] Subsequent addition of 1kg / m to oil-contaminated soil 2 The liquid mixed microbial preparation, the SDS surfactant that a...

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Abstract

The invention discloses a bioremediation method of oil-contaminated soil, and relates to the technical field of oil-contaminated soil remediation. According to the bioremediation method, a microbial remediation and agricultural crop combined remediation technical system is adopted, microbial remediation is firstly adopted, and then agricultural crops are adopted for remediation treatment. The advantages of microbial remediation and phytoremediation are combined, the oil-contaminated soil can be lowered to the low concentration within short time, treatment cost is low, and certain actual application value is achieved.

Description

technical field [0001] The invention relates to the technical field of remediation of petroleum-contaminated soil, in particular to a bioremediation method of petroleum-contaminated soil. Background technique [0002] Oil has caused extensive soil pollution during extraction, transportation and processing. Petroleum hydrocarbons are chemical substances produced by the use of petroleum in different human activities. They are a class of priority toxic organic pollutants that commonly exist in the environment. They are highly persistent and harmful in the environment. Some of these PAHs not only have strong carcinogenic, teratogenic and mutagenic toxicity, but also can be gradually enriched and amplified in animals and plants through the food chain, thereby posing a serious threat to human health. With the rapid expansion of petroleum-contaminated soil, how to restore and control it economically and effectively has become a major technical difficulty in the field of environmen...

Claims

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Application Information

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IPC IPC(8): B09C1/00B09C1/10
CPCB09C1/105B09C2101/00
Inventor 车春玲
Owner JINAN JIAXING TECH
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