Strawberry stem tip detoxification tissue culture method for lowering aberration rate

Abstract

The invention provides a strawberry stem tip detoxification tissue culture method for lowering the aberration rate. The method includes the following steps that a general induction culture medium for different gene types of strawberries is prepared, primary culture is conducted on strawberry stem tips, and a virus-free plant is detected and screened to serve as a multiplication stock plant; a general multiplication culture medium of different gene types of strawberries is prepared; the multiplication stock plant is subjected to multiplication culture; then rooting culture is conducted, and acclimatizating and transplanting are conducted. According to the strawberry stem tip detoxification tissue culture method, a general culture medium formula which is suitable for conducting stem and tip tissue culture on different types of strawberries is determined, the induction culture medium and the first multiplication culture medium are unified into one type, it is unnecessary to screen different culture medium formulas for different varieties, the multiplication culture medium formula lowers the use concentration of cytokinin (6-BA), and thus generation of a great number of weak and small bud plexuses and thin and long and invalid tissue culture seedlings is avoided; besides, an alternate multiplication culture method is adopted, the number of times of secondary culture is controlled to maintain at eight, generation of variation is reduced, and standard production is easy to conduct.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for cultivating virus-free strawberry stem tips with reduced mutation rate. Background technique [0002] Viral diseases are serious hazards in strawberry production, which restricts the development of strawberry production. The cultivation and application of virus-free seedlings is an effective way to solve this problem. Due to the inconsistency of the technical standards for the breeding of virus-free seedlings in various places, the virus-free rate of seedlings bred by tissue culture is not high, the virus has not been detected, the rate of seedling mutation increases, and the quality of seedlings is disordered, which has brought harm to strawberry production. and huge potential risks. The technical problems mainly solved by the invention are: detoxification of shoot tip tissue culture, control of tissue culture variation rate, and rapid propagation of high-quality tiss...

AI Technical Summary

Problems solved by technology

[0005] 1. Strawberry genotypes (variety) are different, and the hormone ratios of the induction medium, proliferation medium, and rooting medium used are different. It is necessary to screen different medium formulas for each variety, which is time-consuming and laborious.

[0006] 2. Shoot tip induction medium and proliferation medium are prepared with different formulations, the procedure is cumbersome and the efficiency is low

[0007] 3. Improper selection of hormone types and concentration ratios in the proliferation medium

The concentration of BA is high, up to 3.0mg / L, resulting in a large number of bud clusters, the proliferation coefficient is too high, the proliferation of seedlings is weak, slender, easy to vitrification, thereby greatly increasing the mutation rate, and the seedling rate is low; use NAA (or 2,4-D) and other auxins, easy to produce callus, thereby increasing the mutation rate

[0008] 4. The number of subcultures is too many, or even uncontrolled for many years, and the subculture is carried out for many years, and the long-term stress culture under the adversity condition of the tissue culture leads to the occurrence of the variation of the tissue culture seedlings