Novel nano-sized artificial red blood cell, artificial blood and preparation method of novel nano-sized artificial red blood cell

A red blood cell and artificial technology, applied in the direction of extracellular fluid diseases, blood diseases, medical preparations with non-active ingredients, etc., can solve the problems of threatening the life of blood transfusions, short blood storage time, easy leakage of hemoglobin, etc., and achieves a simple preparation method Easy to use, good biocompatibility, and good functional stability

Inactive Publication Date: 2016-04-20
SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Clinical blood transfusion has been widely used, but there are still many problems in blood transfusion: 1. Blood may carry pathogens (hepatitis virus, HIV, etc.), and some diseases may be transmitted through blood transfusion; 2. Blood type mismatch can cause agglutination, which can threaten the transfused Human life; 3. Shortage of blood sources; 4. Short storage time of blood (42 days)
Hemoglobin liposomes have poor structural stability in the body, and the encapsulated hemoglobin is easy to leak

Method used

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  • Novel nano-sized artificial red blood cell, artificial blood and preparation method of novel nano-sized artificial red blood cell
  • Novel nano-sized artificial red blood cell, artificial blood and preparation method of novel nano-sized artificial red blood cell
  • Novel nano-sized artificial red blood cell, artificial blood and preparation method of novel nano-sized artificial red blood cell

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Experimental program
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Effect test

Embodiment 1

[0031] Embodiment 1: Preparation of nanoartificial erythrocytes

[0032] PLGA was dissolved in acetonitrile at a concentration of 2 mg / mL; 300 μg lecithin, 200 μg DSPE-PEG (2000), and 1500 μg hemoglobin were added to 3 mL of 4% ethanol aqueous solution; 1 mL of PLGA acetonitrile solution was added dropwise to 4% ethanol aqueous solution, and an ultrasonic cell disruptor was used during the process Sonicate at a frequency of 20 kHz and a power of 35 W for 5 minutes (while monitoring the temperature of the liquid during preparation, and use an ice bath to control the temperature below 40 degrees Celsius to prevent heat denaturation of hemoglobin) to obtain a brown-red solution. Adopt 100kDa ultrafiltration tube 5000r / min centrifugation and ultrafiltration twice (10min each time), and pass through nitrogen for deoxygenation treatment to obtain nano-artificial erythrocytes.

[0033] figure 1 It is a transmission electron micrograph of nano-artificial erythrocytes in the artificia...

Embodiment 2

[0034] Embodiment 2: Preparation of nanoartificial erythrocytes

[0035] PLGA was dissolved in acetonitrile at a concentration of 2 mg / mL; 350 μg lecithin, 200 μg DSPE-PEG (5000), and 2000 μg hemoglobin were added to 3 mL of 4% ethanol aqueous solution; 1 mL of PLGA acetonitrile solution was added dropwise to 4% ethanol aqueous solution, and an ultrasonic cell disruptor was used during the process Sonicate at a frequency of 20 kHz and a power of 35 W for 5 minutes (while monitoring the temperature of the liquid during preparation, and use an ice bath to control the temperature below 40 degrees Celsius to prevent heat denaturation of hemoglobin) to obtain a brown-red solution. Adopt 100kDa ultrafiltration tube 7000r / min centrifugation and ultrafiltration twice (each centrifugation 5min), and pass through nitrogen to do deoxygenation treatment, obtain nanoartificial erythrocytes. The average particle diameter measured by a particle size analyzer is 70nm. In this embodiment, the...

Embodiment 3

[0036] Embodiment 3: Preparation of nanoartificial erythrocytes

[0037] Referring to Example 1, the ultrasonic power was changed to 65W to prepare nano-artificial erythrocytes. The average particle diameter measured by the particle size analyzer is 62nm. In this example, the encapsulation rate of hemoglobin is 76.24±1.77%.

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Abstract

The invention provides a novel nano-sized artificial red blood cell, artificial blood and a preparation method of the novel nano-sized artificial red blood cell. According to the nano-sized artificial red blood cell, hemoglobin is adopted as an oxygen-carrying molecule and is entrapped by a polymer to form a core; a phospholipid molecular layer modified with a polyethylene glycol molecule is adopted outside the core to simulate a cell membrane to protect the hemoglobin molecule. Besides, the size of the nano-sized artificial red blood cell ranges from 50 nm to 200 nm. The nano-sized artificial red blood cell can be synthesized on a large scale, is stable in structure, high in entrapment rate and not likely to leak, has good biocompatibility, structural stability and functional stability in vivo and can convey oxygen to the organism efficiently without causing obvious side effects. The method is simple, easy and convenient to implement and convenient to popularize.

Description

technical field [0001] The invention relates to a novel nano-artificial red blood cell, artificial blood and a preparation method thereof, which are used to solve the problem of oxygen supply in the body. Background technique [0002] Clinical blood transfusion has been widely used, but there are still many problems in blood transfusion: 1. Blood may carry pathogens (hepatitis virus, HIV, etc.), and some diseases may be transmitted through blood transfusion; 2. Blood type mismatch can cause agglutination, which can threaten the transfused 3. Shortage of blood sources; 4. The storage time of blood is short (42 days). Recently, nanotechnology has enabled the rapid development of artificial oxygen carriers, which has attracted widespread attention. Artificial oxygen carriers are nano / micro-sized particles with oxygen-carrying function, which can be synthesized on a large scale without causing cross-infection and agglutination. At present, a variety of artificial oxygen carrie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/42A61K35/14A61K47/34A61K47/24A61P7/08A61K47/10
CPCA61K38/42A61K9/0026A61K35/14A61K47/24A61K47/34
Inventor 蔡林涛罗震宇郑明彬赵鹏飞陈泽倪大鹏
Owner SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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