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Schizophyllum strain and application thereof
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A technology of schizophyllum and microbial strains, applied in the direction of fungi, biochemical equipment and methods, microorganisms, etc., to achieve a significant effect of adsorbing coal powder
Active Publication Date: 2016-04-20
NANJING UNIV
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However, so far, scholars at home and abroad have studied the bio-methanogenic bacteria mainly archaea, and there are very few reports on fungal methanogenesis, especially the conversion of coal to methane under anaerobic conditions.
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Embodiment 1
[0028] 1. Configure culture medium
[0029]1.1 Separation medium: 3g potato powder, 2.5g malt extract, 0.125g beef extract, 1g yeast powder, 1g casein peptone, 1g trisodium D-fructose-1,6-diphosphate, 0.375g sodium acetate, chloramphenicol 500μg, erythromycin 500μg, agar 15g, NaCl27.25g, NaCl 2 SO 4 0.16g, KCl0.11g, NH 4 Cl0.08g, MgCl 2 3.05g, CaCl 2 1.69g, KBr0.08g, H 3 BO 3 0.01g, FeSO 4 0.0005g, ZnSO 4 0.0003g, MnCl 2 0.002g, CuSO 4 0.0005g, distilled water 1L. Adjust the pH to 7.7, and sterilize at 121°C for 20 minutes.
[0030] 1.2 Purification medium: Potato powder 6g, glucose 12g, agar 20g, NaCl27.25g, NaCl 2 SO 4 0.16g, KCl0.11g, NH 4 Cl0.08g, MgCl 2 3.05g, CaCl 2 1.69g, KBr0.08g, H 3 BO 3 0.01g, FeSO 4 0.0005g, ZnSO 4 0.0003g, MnCl 2 0.002g, CuSO 4 0.0005g, distilled water 1L. Natural pH, sterilized at 121°C for 20min.
[0032] Spread a piece of aluminum foil paper on the ultra-clean workbench, spray it with 75% e...
Embodiment 2
[0050] Functions of 15R-5-F01 strain for adsorption of pulverized coal and anaerobic methanogenesis
[0051] 1. Material: Xinjiang lignite SH2030, specular reflectance (R o = 0.49%), crush the lignite block, place it in a clean mortar and grind it fully, and pass through a 200-mesh sieve to collect lignite with a particle size below 200 mesh, and sterilize it in an autoclave at 115°C for 15min.
[0052] 2. Culture medium: 10g of 200 mesh lignite coal powder, 2g of glucose, NH 4 Cl4g, KH 2 PO 4 2g, FeSO 4 0.5mg, ZnSO 4 0.3mg, MnCl 2 2.0mg, CuCl 2 0.5mg, NaCl27.25g, NaCl 2 SO 4 0.16g, KCl0.11g, NH 4 Cl0.08g, MgCl 2 3.05g, CaCl 2 1.69g, KBr0.08g, H 3 BO 3 0.01g, FeSO 4 0.0005g, ZnSO 4 0.0003g, MnCl 2 0.002g, CuSO 4 0.0005g, distilled water 1L. Natural pH, sterilized at 115°C for 20min.
[0053] 3. Pulverized coal adsorption and methane production
[0054] Inoculate fungal hyphae in the center of the purified medium plate, and culture it in a constant temperatu...
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Abstract
The invention particularly relates to a Schizophyllum strain and application thereof. The Schizophyllum strain is collected by General Microorganism Center of China Committee for Culture Collection of Microorganisms on November 24th, 2015, the collection number is CGMCC No.11604, and the collection name is Schizophyllum 15R-5-F01. The Schizophyllum strain provided by the invention is taken from the lignite bed from a 1923-deep marine bottom sediment in Pacific Ocean and prepared by carrying out separation and purification, and is a new strain after being identified. The comprehensive morphological identification and ribosomeRNA (ribonucleic acid) sequential analysis results indicate that the strain 15R-5-F01 is a Schizophyllum strain, and belongs to Schizophyllum, Agaricales and Basidiomycota. The Schizophyllum strain provided by the invention has very obvious coalpowderadsorption effect, and can be used for producing methane by using lignite as a raw material.
Description
technical field [0001] The invention relates to the field, in particular to a schizophyllum and its application. Background technique [0002] At present, air pollution and water pollution are serious problems, especially the content of PM2.5, which poses a great threat to air visibility and human health. PM2.5 refers to particulate matter with a diameter less than or equal to 2.5 microns in the atmosphere, also known as particulate matter that can enter the lungs. The chemical composition mainly includes organic carbon (OC), elemental carbon (EC), nitrate, sulfate, ammonium salt, sodium salt (Na + )Wait. Among them, coalpowder particles produced in engineering operations such as coal mines are also one of the sources of PM2.5 in the atmosphere, and there is no effective solution to the pollution of soil and water by waste coal slag particles generated during operations. [0003] Lignite is the least coalified mineral coal. A brown-black, dull, low-rank coal between pea...
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