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A kind of Schizophyllum and its application
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A technology of Schizophyllum and strains, applied in fungi, biochemical equipment and methods, microorganisms, etc., to achieve the effect of significantly adsorbing pulverized coal
Active Publication Date: 2018-08-14
NANJING UNIV
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However, so far, scholars at home and abroad have studied the bio-methanogenic bacteria mainly archaea, and there are very few reports on fungal methanogenesis, especially the conversion of coal to methane under anaerobic conditions.
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Embodiment 1
[0028] 1. Configure culture medium
[0029]1.1 Separation medium: 3g potato powder, 2.5g malt extract, 0.125g beef extract, 1g yeastpowder, 1g casein peptone, 1g trisodium D-fructose-1,6-diphosphate, 0.375g sodium acetate, chloramphenicol 500μg, erythromycin 500μg, agar 15g, NaCl27.25g, NaCl 2 SO 4 0.16g, KCl 0.11g, NH 4 Cl 0.08g, MgCl 2 3.05g, CaCl 2 1.69g, KBr 0.08g, H 3 BO 3 0.01g, FeSO 4 0.0005g, ZnSO 4 0.0003g, MnCl 2 0.002g, CuSO 4 0.0005g, distilled water 1L. Adjust the pH to 7.7, and sterilize at 121°C for 20 minutes.
[0030] 1.2 Purification medium: Potato powder 6g, glucose 12g, agar 20g, NaCl 27.25g, NaCl 2 SO 4 0.16g, KCl0.11g, NH 4 Cl 0.08g, MgCl 2 3.05g, CaCl 2 1.69g, KBr 0.08g, H 3 BO 3 0.01g, FeSO 4 0.0005g, ZnSO 4 0.0003g, MnCl 2 0.002g, CuSO 4 0.0005g, distilled water 1L. Natural pH, sterilized at 121°C for 20min.
[0032] Spread a piece of aluminum foil paper on the ultra-clean workbenc...
Embodiment 2
[0050] Functions of 15R-5-F01 strain for adsorption of pulverized coal and anaerobic methanogenesis
[0051] 1. Material: Xinjiang lignite SH2030, specular reflectance (R o = 0.49%), crush the lignite block, place it in a clean mortar and grind it fully, and pass through a 200-mesh sieve to collect lignite with a particle size below 200 mesh, and sterilize it in an autoclave at 115°C for 15min.
[0052] 2. Culture medium: 10g of 200 mesh lignite coal powder, 2g of glucose, NH 4 Cl 4g, KH 2 PO 4 2g, FeSO 4 0.5mg, ZnSO 4 0.3mg, MnCl 2 2.0mg, CuCl 2 0.5mg, NaCl 27.25g, NaCl 2 SO 4 0.16g, KCl 0.11g, NH 4 Cl 0.08g, MgCl 2 3.05g, CaCl 2 1.69g, KBr 0.08g, H 3 BO 3 0.01g, FeSO 4 0.0005g, ZnSO 4 0.0003g, MnCl 2 0.002g, CuSO 4 0.0005g, distilled water 1L. Natural pH, sterilized at 115°C for 20min.
[0053] 3. Pulverized coal adsorption and methane production
[0054] Inoculate fungal hyphae in the center of the purified medium plate, and culture it in a co...
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Abstract
The invention relates to the field, in particular to a schizophyllum and its application. A kind of Schizophyllum, preserved in the General Microorganism Center of China Microbiological CultureCollection Management Committee on November 24, 2015, the preservation number is CGMCC NO.11604, and the preservation name is Schizophyllum (Schizophyllum) 15R‑5‑F01. The schizophyllum provided by the invention is obtained by separating and purifying from a lignite layer at a depth of 1923 meters in the Pacific seabedsediment, and is identified as a new bacterial strain. Based on the results of morphological identification and ribosomal RNAsequence analysis, the strain 15R‑5‑F01 was identified as Schizophyllum, belonging to Basidiomycota, Agaricales, and Schizophyllum genus. The schizophyllum provided by the invention has a very remarkable effect of adsorbing coal dust, and can also produce methane by using lignite as a raw material.
Description
technical field [0001] The invention relates to the field, in particular to a schizophyllum and its application. Background technique [0002] At present, air pollution and water pollution are serious problems, especially the content of PM2.5, which poses a great threat to air visibility and human health. PM2.5 refers to particulate matter with a diameter less than or equal to 2.5 microns in the atmosphere, also known as particulate matter that can enter the lungs. The chemical composition mainly includes organic carbon (OC), elemental carbon (EC), nitrate, sulfate, ammonium salt, sodium salt (Na + )Wait. Among them, coalpowder particles produced in engineering operations such as coal mines are also one of the sources of PM2.5 in the atmosphere, and there is no effective solution to the pollution of soil and water by waste coal slag particles generated during operations. [0003] Lignite is the least coalified mineral coal. A brown-black, dull, low-rank coal between pea...
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