Method for evaluating genetic toxicity of drinking water through multi-genetic-end point biological group tests

A genotoxicity, drinking water technology, applied in the direction of testing water, material inspection products, measuring devices, etc., can solve the problems of drinking water safety misjudgment, false negative, affecting the quality of human gene pool, etc., to achieve good versatility and detection means easy effect

Inactive Publication Date: 2016-04-20
HARBIN INST OF TECH
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Due to the individual sensitivity and specificity of the tested cells or animals to toxic pollutants, false negative results

Method used

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  • Method for evaluating genetic toxicity of drinking water through multi-genetic-end point biological group tests
  • Method for evaluating genetic toxicity of drinking water through multi-genetic-end point biological group tests
  • Method for evaluating genetic toxicity of drinking water through multi-genetic-end point biological group tests

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Experimental program
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specific Embodiment approach 1

[0015] Specific implementation mode 1: This implementation mode provides a method covering multiple genetic endpoints to evaluate the genotoxicity of drinking water, and its technical measures adopt the following scheme:

[0016] Although there are many methods for detecting genotoxicity at present, they can be roughly divided into the following three categories in terms of detection methods: gene mutation test, chromosome aberration test, and DNA damage and repair test. The selection of mutagenesis tests should meet the following principles: ①It should include multiple genetic endpoints; ②Test organisms include prokaryotic, eukaryotic and mammalian species; ③Include in vivo and in vitro tests; ④Include sex cells and somatic cells; ⑤Detection methods Relatively simple, fast, economical and good laboratory versatility and other principles. In addition, the weight value of the mutagenic test evaluation results follows the following principles: (1) The degree of evolution of the ...

specific Embodiment approach 2

[0033] Specific implementation mode 2: In this implementation mode, a waterworks in the south of China is evaluated. The effluent from the filter of the water intake plant and the disinfected water were evaluated for genotoxicity according to the method described in Embodiment 1.

[0034] 1. Ames test analysis

[0035] The histidine-deficient Salmonella typhimurium (TA98 and TA100 strains) used in this test were directly provided by the United States University of California Ames laboratory, and the characteristics of the identified strains met the test requirements.

[0036] Count the number of back-changing colonies in each plate, and calculate the mean number and standard deviation of 6 parallel plates to obtain the mutagenic rates MR of TA98 and TA100 respectively. The results are shown in Table 1. It is generally believed that the criteria for judging a positive Ames test (gene mutation that can cause base substitution type) must meet two conditions at the same time: ① T...

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Abstract

The invention discloses a method for evaluating the genetic toxicity of drinking water through multi-genetic-end point biological group tests. According to the method, water supply plant filter outflow water and disinfected water serve as research objects, biological group genetic toxicity tests: an Ames test, an in vivo micronucleus test in mouse bone marrow polychromatic erythrocyte, a mouse sperm shape abnormality test, a hamster ovary cell micronucleus test and an SOS/umu test serve as evaluation standards, and when 3 positive results exist in the biological group genetic toxicity test, it is shown that biological genetic toxicity safety exists. The method has the following advantages that three genetic end points of gene mutation, chromosomal aberration and DNA damage are contained; test organisms comprise pronuclei, eukaryon and mammals; in-vivo and in-vitro tests are included; sexual cells and body cells are included; the detection means is relatively simple, convenient, rapid, economical and good in laboratory universality.

Description

technical field [0001] The invention belongs to the field of genotoxicity detection and evaluation of drinking water, and relates to a method for accurately evaluating genotoxicity of drinking water by biological group test. Background technique [0002] With the diversification of high-tech industries, various types of refractory new pollutants are discharged into drinking water sources, which poses a potential threat to drinking water safety. Although drinking water technology has been greatly developed, it is difficult to remove organic substances such as nano-sized, trace, and hydrophilic. Such substances jointly act on organisms through synergistic and additive effects, so physical and chemical analysis methods cannot reflect the comprehensive toxic effects and long-term effects of various pollutants on organisms. Another study shows that the mutagenicity of drinking water is related to TOC or COD Mn There is no corresponding relationship among other indicators. Takin...

Claims

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Application Information

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IPC IPC(8): G01N33/18
CPCG01N33/186G01N33/1866
Inventor 徐勇鹏崔福义陈停
Owner HARBIN INST OF TECH
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