Method for detecting ER gene of peripheral blood circulating tumor cells of patient suffering from advanced breast cancer

Abstract

The invention discloses a method for detecting an ER gene of peripheral blood circulating tumor cells of a patient suffering from advanced breast cancer. The method comprises the steps of conducting separation with a membrane filtration device, so that CTC in peripheral blood of the patient suffering from advanced breast cancer is obtained; identifying the peripheral blood circulating tumor cells of the patient suffering from advanced breast cancer through the cellular immunofluorescence technique; preparing thin layer sections through the cell paraffin block technique; further detecting the ER expression condition of the peripheral blood circulating tumor cells of the patient suffering from advanced breast cancer through the immunohistochemical technique. According to the method, CTCs are separated and enriched by means of the ISET technique, and the CTCs are identified through the cellular immunofluorescence technique, so that the difficulty existing in CTCs identification conducted through the pure ISET technique and false negativeness existing in CTCs identification conducted through the pure immunological detection technique are overcome. The technique is not high in equipment requirement, the method is easy to grasp, and real-time monitoring can be conducted. By means of the technical method, the ER expression condition of the patient suffering from advanced breast cancer can be detected without picking breast cancer tissues.

Description

technical field [0001] The invention relates to a new method for detecting ER gene, in particular to a method for detecting ER gene in patients with advanced breast cancer through peripheral blood. Background technique [0002] Estrogen receptor (Estrogen receptor, ER), which is currently included in treatment guidelines as a prognostic factor, has become the most important indicator when formulating endocrine therapy for breast cancer patients. Existing detection of breast cancer ER needs to obtain tumor tissue samples of primary tumor or metastatic tumor through surgery or biopsy. However, not every patient can obtain tumor tissue samples, and continuous monitoring cannot be performed during treatment detection, which limits its use as an effective efficacy monitoring indicator. However, circulating tumor cells shed into the blood can provide us with potential real-time tumor samples, and their mutation status can also be used to guide treatment. [0003] Circulating tum...

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the shortcomings of the traditional circulating tumor cell detection technology and the unsuitable tissue specimen collection for patients with advanced breast cancer-thereby inability to evaluate the ER status of patients, the patent of the present invention provides a detection method for ER gene of circulating tumor cells in peripheral blood of patients with advanced breast cancer: using Separation and acquisition of CTCs in peripheral blood of patients with advanced breast cancer by membrane filtration device; identification of circulating tumor cells in peripheral blood of patients with advanced breast cancer by cell immunofluorescence technology; use of cell wax block technology to make thin-layer sections; and detection of advanced breast cancer by immunohistochemistry ER expression of circulating tumor cells in peripheral blood of patients

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