Cancer cell or bacterium capturing method and kit thereof

Abstract

The invention relates to the field of biomedicine, in particular to a cancer cell or bacterium capturing method and a kit thereof. The method comprises the following steps: co-breeding a cancer cell or bacterium sample with an NTA functional carrier, target binding protein and Ni2+, so that the efficient capturing of cancer cells or bacteria is achieved; and in addition, co-breeding the NTA functional carrier which captures the cancer cells or bacteria with a metal ion chelating agent, so that the controlled release of the cancer cells or bacteria is easily achieved. The method provided by the invention is strong in specificity and high in sensitivity, and is capable of achieving the capturing and the release of the cancer cells or bacteria conveniently, rapidly and reversibly; and in addition, the capturing method is relatively low in influence on the bioactivity of the cancer cells or bacteria.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a method for capturing cancer cells or bacteria and a kit thereof. Background technique [0002] In the field of biomedicine, cancer cells and pathogenic bacteria are two very important biological targets. The high-sensitivity analytical detection of the two is of great significance for the early diagnosis of related diseases (cancer and infectious diseases caused by pathogenic bacteria). However, in most cases, the concentration of cancer cells and pathogenic bacteria in the sample to be tested is relatively low (trace level), and it is difficult to realize direct biochemical analysis and genotyping identification. Therefore, in the actual detection process of cancer cells and pathogenic bacteria, it is necessary to go through a "capture-release" process. First, the target to be tested is effectively captured to achieve its separation and enrichment, and then the enriched target is r...

AI Technical Summary

Problems solved by technology

[0004] However, DNA aptamer method, temperature control method and microfluidic method all have their own shortcomings.

The DNA aptamer method cannot achieve reversible target capture and controllable release, and can only meet the requirements of specific capture and release of cancer cells and pathogenic bacteria for a single time

The temperature-controlled method involves complex thermosensitive polymer synthesis steps in which organic molecules used have certain effects on the activity of biological targets, thereby reducing the final detection accuracy and sensitivity of cancer cells and pathogenic bacteria

The microfluidic method needs to precisely control the structure of the microfluidic capture chip according to the size of different targets, the preparation process is complicated, and the target release process based on microfluidic shear force will reduce the activity of cancer cells and pathogenic bacteria