Anti-gonyautoxin GTX2,3 monoclonal antibody immuno-colloidal gold labeled test strip and preparation method thereof
A technology of gonitoxin and monoclonal antibody, which is applied in biological testing, material inspection products, measuring devices, etc., can solve the problems of difficult preparation of artificial antigens and difficult realization of monoclonal antibody gold standard test strips, etc., and achieve a good society And economic value, easy to promote and apply, good stability
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[0023] Example 1
[0024] An immune colloidal gold-labeled test strip with monoclonal antibodies against Glycotoxin GTX2,3. The bottom layer of the test strip is a support layer, and the middle layer is an adsorption layer. The protective layer is fixed on the adsorption layer. The test ends are the absorbent fiber layer, the nitrocellulose membrane layer, and the water-absorbing material layer at the end. The gold-labeled antibody in the gold-labeled antibody fiber layer is an anti-GTX toxin monoclonal antibody; on the nitrocellulose membrane layer, there is a Gnocchia The toxin has a control line (C) and a detection line (T).
[0025] A preparation method of immunogold-labeled test strips for anti-Gynophyllotoxin GTX2,3 monoclonal antibody, including coating of antigen GTX2,3-Gly-KLH and preparation of monoclonal antibody of Glyotoxin GTX2,3 Preparation, preparation of colloidal gold, preparation of colloidal gold labeled antibody and test paper:
[0026] Preparation of monoclona...
Example Embodiment
[0031] Example 2
[0032] An immune colloidal gold-labeled test strip with monoclonal antibodies against Glycotoxin GTX2,3. The bottom layer of the test strip is a support layer, and the middle layer is an adsorption layer. The protective layer is fixed on the adsorption layer. The test ends are the absorbent fiber layer, the nitrocellulose membrane layer, and the water-absorbing material layer at the end. The gold-labeled antibody in the gold-labeled antibody fiber layer is an anti-GTX toxin monoclonal antibody; on the nitrocellulose membrane layer, there is a Gnocchia The toxin has a control line (C) and a detection line (T).
[0033] A preparation method of immunogold-labeled test strips for anti-Gynophyllotoxin GTX2,3 monoclonal antibody, including coating of antigen GTX2,3-Gly-KLH and preparation of monoclonal antibody of Glyotoxin GTX2,3 Preparation, preparation of colloidal gold, preparation of colloidal gold labeled antibody and test paper:
[0034] Preparation of monoclona...
Example Embodiment
[0039] Example 3
[0040] An immune colloidal gold-labeled test strip with monoclonal antibodies against Glycotoxin GTX2,3. The bottom layer of the test strip is a support layer, and the middle layer is an adsorption layer. The protective layer is fixed on the adsorption layer. The test ends are the absorbent fiber layer, the nitrocellulose membrane layer, and the water-absorbing material layer at the end. The gold-labeled antibody in the gold-labeled antibody fiber layer is an anti-GTX toxin monoclonal antibody; on the nitrocellulose membrane layer, there is a Gnocchia The toxin has a control line (C) and a detection line (T).
[0041] A preparation method of immunogold-labeled test strips for anti-Gynophyllotoxin GTX2,3 monoclonal antibody, including coating of antigen GTX2,3-Gly-KLH and preparation of monoclonal antibody of Glyotoxin GTX2,3 Preparation, preparation of colloidal gold, preparation of colloidal gold labeled antibody and test paper:
[0042] Preparation of monoclona...
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