Anti-gonyautoxin GTX2,3 monoclonal antibody immuno-colloidal gold labeled test strip and preparation method thereof

Abstract

The invention relates to an anti-gonyautoxin GTX2,3 monoclonal antibody immuno-colloidal gold labeled test strip and its preparation method, and belongs to the field of rapid detection technology of saxitoxin. By the application of GTX2,3 artificial antigen prepared by a new method, GTX2,3 monoclonal antibody is obtained. According to the test strip, a bottom layer is a support layer, an intermediate layer is an adsorption layer, and a protective layer is fixed on the adsorption layer. Starting from a test end, the adsorption layer successively comprises an adsorptive fiber layer, a nitrocellulose membrane layer and a water absorbing material layer at the tail end. Gold-labelled antibody in the gold-labeled antibody fiber layer is anti-GTX monoclonal antibody. A gonyautoxin control line and a detection line are arranged on the nitrocellulose membrane layer. The preparation method comprises the following steps: coating of antigen GTX2,3-Gly-KLH, preparation of the monoclonal antibody of gonyautoxin GTX2,3, preparation of colloidal gold, colloidal gold labeled antibody, preparation of the test strip and the like. The test strip is prepared by the use of the monoclonal antibody obtained by the new GTX2.3 artificial antigen preparation method. The test strip has advantages of fast, accurate and simple detection, high sensitivity and field testing.

Description

technical field [0001] The invention relates to an immune colloidal gold-labeled test strip for resisting gonylin GTX2,3 monoclonal antibodies and a preparation method thereof, belonging to the technical field of rapid detection of shellfish toxin monoclonal antibodies. Background technique [0002] In recent years, due to the eutrophication of water bodies, algae proliferate abnormally and release algae toxins (AlgaeToxin). These secondary metabolites seriously endanger the safety of human water and the safety of other aquatic organisms. GTX The resulting paralytic shellfish toxin has attracted more attention. GTX2,3 is one of more than 60 isomers of gonylins that have been discovered, and it is one of the main components of the paralytic shellfish toxins of poisoned shellfish and toxic red tide algae in the southern coastal areas of my country. It is characterized by relatively small molecules, high toxicity, serious harm to the human body, and lack of complete antigenic p...

AI Technical Summary

Problems solved by technology

[0007] The present invention aims at the problem in the prior art that it is difficult to prepare the artificial antigen of the small molecule gonyellin GTX2,3, so it is difficult to realize the gold standard test strip of its monoclonal antibody. The GTX2,3 artificial antigen prepared by the inventor is used to obtain GTX2 ,3 monoclonal antibody, providing an immune colloidal gold-labeled test strip against gonylin GTX2,3 monoclonal antibody, which is aimed at the marine environment, especially the increasingly serious pollution of the southern coastal waters, which leads to GTX shellfish toxin in shellfish and crabs, etc. Aquatic organisms accumulate in the body and cause harm to the human body. A simple, fast and accurate on-site detection method is urgently needed. This invention is designed and invented based on the physiological characteristics of aquatic organisms such as shellfish and crabs, and is prepared by using the principle of indirect ELISA competition. , in order to achieve fast, accurate, simple, high sensitivity, on-site detection purposes

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