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Bioremediation agent for treating compound contaminated soil and application thereof

A bioremediation agent and compound pollution technology, which is applied in the field of soil heavy metal pollution restoration, can solve the problem that the restoration of compound heavy metal contaminated soil cannot meet the standard requirements, and achieve the effect of enhancing soil fertility

Active Publication Date: 2016-05-04
山东君德生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the compound conditioner and repair method in this invention have a good fixation effect on cadmium or heavy metals with similar properties, but cannot meet the standard requirements for the repair of compound heavy metal contaminated soil.
However, the coexistence of various heavy metal pollutions in soil is serious, so there is a need for a method that can effectively remediate compound heavy metal contaminated soil.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] (1) Liquid fermentation

[0062] 1. Liquid seed preparation of Bacillus megaterium:

[0063] ①Strain activation

[0064] The Bacillus megaterium strains preserved in the freeze-drying tube were aseptically inserted into the nutrient broth agar medium, and cultivated at a constant temperature of 30°C.

[0065] ② Shake flask liquid culture

[0066] Using nutrient gravy liquid medium, under the conditions of 150r / min, 30°C, and 20h, 2500mL of shake flask seed liquid was obtained.

[0067] ③Seed tank cultivation

[0068] Nutrient gravy medium is used, the volume of the seed tank is 100L, the filling ratio is 50%, the inoculum size is 5%, the air volume is 2VVmm [the air volume (m3) under the standard state of the fermentation broth volume (m3) per unit time (min) ], under the conditions of 150r / min, 30°C, and 16h, a 50L seed tank culture solution was prepared, and the number of viable bacteria was 305×10 8 pcs / g.

[0069] 2. Liquid seed preparation of Agrococcus:

[...

Embodiment 2

[0100] (1) Liquid fermentation

[0101] The preparation method of the liquid seeds of Bacillus megaterium, the liquid seeds of Agrococcus and the liquid seeds of Providencia is the same as in Example 1.

[0102] When preparing the mixed seed liquid, the prepared liquid seeds of Bacillus megaterium 10L, liquid seeds of Agrococcus 15L and liquid seeds of Providencia 5L were mixed evenly to obtain 30L of mixed seed liquid, and the total number of live bacteria was 238×10 8 per gram, of which the number of viable Bacillus megaterium is 102.7×10 8 Each / gram, the number of Viable bacteria of Providencia 104.2×10 8 Individual / gram, Agrococcus viable count 30.2×10 8 pcs / g.

[0103] (2) The preparation process of the solid-state mixed fermentation medium is the same as in Example 1.

[0104] (3) Solid-state mixed fermentation culture, the process of preparing microbial inoculum is the same as that of Example 1.

[0105] (4) Preparation of bioremediation agent The formula and prepa...

Embodiment 3

[0108] (1) Liquid fermentation

[0109] The preparation method of the liquid seeds of Bacillus megaterium, the liquid seeds of Agrococcus and the liquid seeds of Providencia is the same as in Example 1.

[0110] When preparing mixed seed liquid, the liquid seed 10L of prepared Bacillus megaterium, the liquid seed 10L of Agrococcus and the liquid seed 10L of Providence bacterium are mixed evenly to obtain mixed seed liquid 30L, and the total number of live bacteria is 246×10 8 pc / g, of which the number of viable Bacillus megaterium is 103.5×10 8 pcs / g, the number of Viable bacteria of Providencia 106×10 8 Individual / gram, Agrococcus viable count 36.5×10 8 pcs / g.

[0111] (2) The preparation process of the solid-state mixed fermentation medium is the same as in Example 1.

[0112] (3) Solid-state mixed fermentation culture, the process of preparing microbial inoculum is the same as that of Example 1.

[0113] (4) Preparation of bioremediation agent

[0114] formula:

[01...

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Abstract

The invention discloses a bioremediation agent for treating compound contaminated soil and application thereof, and belongs to the technical field of remediation of heavy metal contaminated soil. The bioremediation agent is formed by mixing 10-35 parts of microbial agents, 5-25 parts of nanoscale shell powder, 2-10 parts of nanometer activated silicon fertilizer and 40-55 parts of aronia melanocarpa fruit residue powder by weight. According to the application method for the bioremediation agent, the bioremediation agent is applied to acid farmland soil contaminated by heavy metal, wherein the heavy metal comprises lead, cadmium and zinc, the concentration of the lead is 250-300 mg / Kg, the concentration of the cadmium is 0.3-0.4 mg / Kg, and the concentration of the zinc is 200-300 mg / Kg. he bioremediation agent is low in price of raw material and easy to apply and operate, can avoid secondary pollution to the farmland soil, improve the soil fertility and promote crop growth, and is high in application and popularization value.

Description

technical field [0001] The invention relates to the technical field of remediation of soil heavy metal pollution, and specifically provides a bioremediation agent for treating compound polluted soil and an application thereof. Background technique [0002] On April 18, 2014, the Ministry of Environmental Protection and the Ministry of Land and Resources issued a survey bulletin showing that the overall soil environment in the country is not optimistic. Some areas have serious soil pollution, the soil environment quality of cultivated land is worrying, and the soil environment problems of industrial and mining wasteland are prominent. Due to the complexity of formation, long-term accumulation, concealment of occurrence, slow effect of toxicity, specificity of diagnosis and long-term recovery of heavy metal pollution in farmland, heavy metal pollution has become an "invisible killer" of human health. On the whole, the complex pollution of various heavy metal elements, such as ...

Claims

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Application Information

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IPC IPC(8): B09C1/10B09C1/08
CPCB09C1/08B09C1/105B09C2101/00
Inventor 杨晓梅周志强常钟周波钱朋智李在贞史晓洁赵传涛蔡洋侯大彬
Owner 山东君德生物科技有限公司
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