Application of anti-glyphosate-related proteins in the regulation of biological glyphosate resistance
A technology for glyphosate resistance and related proteins, which can be used in applications, microorganisms, biochemical equipment and methods, etc., and can solve problems such as rare
Active Publication Date: 2019-05-21
NORTHEAST AGRICULTURAL UNIVERSITY
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Problems solved by technology
However, there are very few reports on other genes related to glyphosate resistance except the EPSPS gene
Method used
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Embodiment 1
[0082] Example 1, the application of anti-glyphosate-related proteins in improving the glyphosate resistance of Escherichia coli
[0083] This embodiment provides the anti-glyphosate-related protein derived from Aspergillus oryzae (Aspergillus oryzae) and related to glyphosate resistance, its name is Mfs40, the amino acid sequence is as shown in sequence 2 in the sequence table, from sequence 1 (sequence The first position of 1 is the 5' terminal nucleotide, and the last position is the 3' terminal nucleotide) to encode the Mfs40 gene.
[0084] 1. Construction of recombinant vector
[0085] At the 5' end of the Mfs40 gene shown in sequence 1, the recognition fragment of EcoR I and the protection base are added, and the recognition fragment of Sma I and the protection base are added at the 3' end to obtain a DNA fragment, which is named as DNA fragment 1 . The DNA fragment 1 was double-digested with restriction endonucleases EcoR I and Sma I to obtain the digested DNA fragmen...
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Abstract
The invention discloses an application of a glyphosate-resistant related protein to regulation and control of glyphosate resistance of an organism. In the application of the glyphosate-resistant related protein to the regulation and control of the glyphosate resistance of the organism, the glyphosate-resistant related protein is shown as (A1), (A2) or (A3), wherein (A1), an amino acid sequence is a protein shown as a sequence 2; (A2) substitution and / or deletion and / or addition of one or more amino acid residues is performed in the amino acid sequence shown as the sequence 2 to obtain a protein with the same function derived from (A1); (A3), a tag is connected to an N end or / C end of the (A1) or the (A2) to obtain a fused protein. As proved by an experiment, the resistance of the organism to the glyphosate can be enhanced through the glyphosate-resistant related protein and an encoding gene thereof, and the glyphosate-resistant related protein and the encoding gene thereof can be used for cultivating high-glyphosate-resistance transgenic crop varieties.
Description
technical field [0001] The invention relates to the application of an anti-glyphosate-related protein in regulating biological glyphosate resistance in the field of biotechnology. Background technique [0002] The use of herbicides is the guarantee of a good agricultural harvest, and has the advantages of fast, efficient and economical. Since the advent of chemical herbicides, there have been more and more varieties and wider application ranges. At present, they are used in almost all kinds of crops everywhere. However, many herbicides have certain limitations in the application process. For example, some herbicides Herbicides have a narrow herbicidal spectrum and are generally effective in controlling weeds; some herbicides are less safe and highly toxic, and are likely to cause soil and water pollution; Agricultural production brings huge losses; the emergence of resistant weeds is accelerated, and few herbicides can truly achieve high efficiency, low toxicity and low res...
Claims
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IPC IPC(8): C07K14/38C12N15/31C12N15/70
CPCC07K14/38C12N2800/101
Inventor 陶波王小琴常淑君邱丽娟田立娟刘洋
Owner NORTHEAST AGRICULTURAL UNIVERSITY