tp recombinant antigen and preparation method and application thereof

Abstract

The invention discloses a TP recombinant antigen and a preparing method and application thereof. The TP recombinant antigen comprises an embedded expression polypeptide and flexible linked peptide favorable for soluble expression connected in sequence; the embedded expression polypeptide is TPN15-TPN17-TPN47 embedded expression polypeptide. The TP recombinant antigen modifies the TPN15-TPN17-TPN47 embedded expression polypeptide by introducing the flexible linked peptide favorable for soluble expression, and the protein activity of the TP recombinant antigen is better compared with that of a traditional TP antigen.

Description

technical field [0001] The invention relates to the field of immune detection, in particular to a TP recombinant antigen and its preparation method and application. Background technique [0002] Syphilis is a sexually transmitted disease, and its pathogen is Treponema pallidum, also called Treponema pallidum (TP), which is mainly transmitted through sexual contact and blood, and produces a variety of symptoms and signs, which appear from time to time, and the course of the disease can vary. It lasts for a long time and can almost invade all organs of the body. It is a sexually transmitted disease that is second only to AIDS and seriously endangers the health of our people. It has been on the rise in my country in recent years. According to the 2014 syphilis key epidemic report, syphilis has ranked third in the number of reported incidences of Class B infectious diseases since 2009. ; Especially in the antenatal outpatient screening of pregnant women positive for syphilis ant...

AI Technical Summary

Problems solved by technology

[0007] 1. When the markers are small markers such as enzymes, luminescent substances, radioactive substances, etc., in order to improve the sensitivity, it is necessary to increase the labeling ratio, but if the ratio is too high, the labeled antigen will be wrapped by the marker, so that the antigenic epitope will be embedded. lead to a decrease in antigenic activity;

[0008] 2. When the marker is a larger nanoparticle such as colloidal gold, latex or other nanoparticle markers, the theoretically optimal sensitivity state is that the lower the labeling ratio, the higher the sensitivity, and the sensitivity is when the molar ratio is 1:1 The highest, but due to the low amount of labeled antigen in the direct labeling process, it will lead to difficult labeling factors such as labeling precipitation, so that the labeling ratio cannot be reduced too low, resulting in low sensitivity, and the increase in the amount of labeled antigen will also lead to specificity reduce

However, prokaryotic expression lacks the post-translational modification function, and sometimes it needs to be expressed together with a special protein partner and further renaturation treatment or storage buffer is explored to make the protein activity better.

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