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Fumonisin b 1 Screening and Application of Nucleic Aptamer Strand Displacement Probes

A nucleic acid aptamer and fumonisin technology, which is applied in DNA/RNA fragments, biochemical equipment and methods, biological testing, etc., can solve the problems of few application reports of nucleic acid aptamers, and achieve low synthesis cost and cost reduction. , good specificity effect

Active Publication Date: 2019-07-05
河南省农业科学院农业质量标准与检测技术研究所
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  • Claims
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Problems solved by technology

In 2010, Maureen McKeague reported on fumonisin B 1 aptamer (Int.J.Mol.Sci.2010, 11:4864-4881; doi:10.3390 / ijms11124864), but there are few reports on the application of this aptamer

Method used

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  • Fumonisin b <sub>1</sub> Screening and Application of Nucleic Aptamer Strand Displacement Probes
  • Fumonisin b <sub>1</sub> Screening and Application of Nucleic Aptamer Strand Displacement Probes
  • Fumonisin b <sub>1</sub> Screening and Application of Nucleic Aptamer Strand Displacement Probes

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Embodiment Construction

[0024] a fumonisin B 1 The nucleic acid aptamer strand displacement probe, the displacement probe is a single-stranded oligonucleotide, the sequence of which is shown in SEQ ID NO:1.

[0025] The 5' end of the probe is modified with a FAM fluorescent group.

[0026] The probe and fumonisin B 1 Aptamers are complementary.

[0027] The fumonisin B 1 The nucleic acid aptamer sequence is shown in SEQ ID NO:6.

[0028] The fumonisin B 1 Aptamer strand displacement probes were screened by fluorescence spectroscopy and fluorescence polarization.

[0029] Fumonisin B 1 Nucleic Aptamer Strand Displacement Probe as Fumonisin B in Maize and Wheat 1 Fluorescence polarization detection analysis applications.

[0030] Below in conjunction with specific embodiment, the present invention is described in detail, and concrete steps are as follows:

[0031] FB used in the present invention 1 The nucleic acid aptamer sequence is FB reported by Maureen McKeague in 2010 1 The sequence of...

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Abstract

The invention belongs to the biotechnological field, and relates to application and development of aptamers, in particular to screening and application of a fumonisin B1 aptamer strand displacement probe. Through screening of the FB1 aptamer strand displacement probe, a foundation is laid for research and development of a subsequent signal amplification sensor technique based on the FB1 aptamer strand displacement probe. An FB1 fluorescence polarization detection technique based on the strand displacement probe adopts an FB1 aptomer as a molecular recognition element and has the advantages of being low in cost and high in specificity compared with an immunodetection technique based on an antibody; in addition, the technique is a homogeneous-phase detection technique, the repeatability is good, operation is easy and convenient, parallel detection of a large quantity of samples can be achieved, building of an agricultural product fumonisin monitoring technique system suitable for the national conditions of China is facilitated, the urgent need of China for a key agricultural product fumonisin pollution monitoring technique is met, the important significance on ensuring the agricultural product consumption safety and life and health of people is achieved, and the application prospect is wide.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to the application and development of nucleic acid aptamers, specifically fumonisin B 1 Screening and application of nucleic acid aptamer strand displacement probes. Background technique [0002] Fumonisins are a class of mycotoxins mainly produced by Fusarium moniliforme. Fumonisins mainly exist in corn and its products, in addition to rice, wheat, barley, sorghum, peas, asparagus, beer, milk and feed and other agricultural products and their processed products. Surveys show that corn and its products from all over the world are generally contaminated with fumonisins, whether it is food for humans or feed for animals, and the pollution rate of corn is more than 60%. At least 15 fumonisin analogues have been identified, of which fumonisin B 1 (FB 1 ) is the most toxic. Studies have confirmed that fumonisins can cause equine leukomalacia and porcine pulmonary edema syndrome. The Worl...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/115G01N33/68
CPCC12N15/115C12N2310/16G01N33/68
Inventor 刘继红王红旗李淑芳尹海燕马莹李静祁玉峰张军锋
Owner 河南省农业科学院农业质量标准与检测技术研究所
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